Team:Imperial College London/Project Chemotaxis Modelling
From 2011.igem.org
Module 1: Phyto-Route
Chemotaxis is the movement of bacteria based on attraction or repulsion of chemicals. Roots secrete a variety of compounds that E. coli are not attracted to naturally. Accordingly, we engineered a chemoreceptor into our chassis that can sense malate, a common root exudate, so that it can swim towards the root. Additionally, E. coli are actively taken up by plant roots, which will allow targeted IAA delivery into roots by our system.
Modelling
Chemotaxis is the movement of bacteria up a concentration gradient of chemoattractants (e.g. malate in our project) and away from repellents (e.g. poisons). E.coli is too small to detect any concentration gradient between its two ends.They resample their surroundings every 3-4 seconds, and then decide whether to tumble or to run[1]. Chemoattractant transiently increases the probability of ‘tumbling’ (or bias), and then a sensory adaptation process returns the bias to baseline, enabling the cell to detect and respond to further concentration changes. The response to a small step change in chemoattractant concentration in a spatially uniform environment increases the response time from 1 second to 2-4 seconds [2]. Changes to saturating levels of chemoattractant can increase the response time to several minutes.
Each chemoreceptor on the bacterium has a periplasmic binding domain and a cytoplasmic signalling domain that communicate with the flagellar motors via a phospho-relay sequence involving the CheA, CheY, and CheZ proteins. The results of modelling the chemotaxis pathway will determine the threshold chemoattractant concentration for bacterial detection and the level where the bacterial chemoattractant detection becomes saturated and bacterial response to chemoattractant becomes less efficient.
2.1 Objective
Use MATLAB to model the chemotaxis pathway and thereby determine the detection threshold and saturation level of chemoattractant for bacteria with 8uM chemoreceptor. As it is believed that the auxin should be placed close to the seed (<0.25 cm [4]), it is crucial to determine whether our bacteria will be able to stay close to the seed.
2.2 Description
Figure 1[1]: Chemotaxis signaling components and pathways for E.coli.
The chemotaxis pathway in E. coli is demonstrated in Figure 1. Chemoreceptors form stable ternary complexes with the CheA and CheW proteins to generate signals that control the direction of rotation of the flagellar motors [5]. The signalling groups currency is in the form of phosphoryl (p), made available to the CheY and CheB effector proteins through autophosphorylation of CheA [1]. CheYp initiates flagellar responses by interacting with the motor to enhance the probability of ‘run’ [1]. CheBp is part of a sensory adaptation circuit that terminates motor responses [1]. Therefore, studying of methylation level, phosphorylation level of CheB and CheY are important to understand chemotaxis of a single cell. The model based on Spiro et al. (1997) [1] was used to study how the concentrations of proteins in the chemotaxis pathway change over time.
In addition, the quantity that links the CheYp concentration with the type of motion (run vs. tumble) is called bias. It is defined as the fraction of time spent on the run with respect to the total movement time. The relative concentration of CheYp is converted into motor bias using a Hill function (Euqation 1), CheYpwt is defined as wild type CheYp[5].
2.3 Results and discussion
Based on the Spiro model, the methylation level of receptors, phosphorylation level of CheY and CheB were studied from Spiro’s model (Figure 2).
Fig.2(a): The phosphorylation level of CheY. Fig.2(b): Phosphorylation of CheB.Fig.2(c): Methylation level of Chemoreceptor. Fig.2(d): The probablity of of bacteria in the running state at different levels of CheYp. Fig.2 (a)(b)(c) show that the threshold detection concentration is 10-8M, and the saturation concentration is 10-5M.
3.1 Objective
Model the chemotaxis of bacterial population dynamics under experimental and natural conditions. The model under laboratory conditions will aid wet lab in designing their experiments. And the model under real soil condition will further inform our project about how and where we can place our bacteria.
Under experimental conditions, the chemoattractant diffuses all the time from the source. However, in real soil, the root produces malate all the time, therefore we assume that the distribution of chemoattractant outside the seed is steady and time-independent. Hence, the modelling of bacterial population chemotaxis will be built with different patterns of chemoattractant distribution.
3.2 Description
This part of modelling focused on creating the movement model of a bacterial population for chemotaxis. In chemotaxis, chemoreceptors sense an increase in the concentration of chemoattractant and then send a signal that suppresses tumbling, while simultaneously, the receptor becomes more methylated. Conversely, a decrease in the chemoattractant concentration increases the tumbling frequency and causes receptor demethylation. The tumbling frequency is approximately 1 Hz when flagellar movement is unbiased, and decreases to almost zero as the bacteria move up a chemotatic gradient [5]. In order to accurately built this model, the following assumptions were made based on literature:
1) During the directed movement phase, the mean velocity at which E. coli moves is 24.1 μm/s [7]. Whereas during the tumbling phase, the speed is insignificantly slower and can be neglected.
2) E. coli usually takes the previous second as their basis on deciding whether the concentration has increased or not. Therefore, in our model the bacteria will be able to compare the concentration of chemoattractant at t seconds to that at t-1 seconds.
3) In our model, we ignored that E. coli do not travel in a straight line during a run, but take curved paths due to unequal firing of flagella.
4) Our model did not consider the growth of bacteria or the tendency of bacteria to congregate into a small area due to quorum sensing.
In the model, the bacteria should be able to compare the chemoattractant concentration at the current time point to the concentration at the previous second. If the concentration decreases (i.e. Ct1-Ct2 ≤0), the bacteria will tumble with a frequency of 1 Hz. If the concentration increases (Ct1-Ct2>0), the tumbling frequency decreases, hence the probability of tumbling decreases. From equation 10 in ref [6], we know that when Ct1-Ct2 >0, the probability of tumbling decreases as an exponential function of chemostatic constant, bacterial velocity , concentration difference between adjacent time points and angle between the two time points. Therefore, we can conclude the above description into the following statement [8]:
Under laboratory conditions, the chemoattractant continuously diffuses from the source, hence the distribution pattern of chemoattratctant changes with time. In this case, the error function (Equation 2) was used to describe the non-steady state chemoattractant distribution.
However, in real soil, malate is used as the chemoattractant. Malate is constantly secreted from the root tip at a concentration of 3 mM [9]. In this case, the malate source is always replenished due to continuous secretion from the root and the distribution pattern can be considered as steady (i.e. independent of time). The steady-state Keler-Segel model was used to demonstrate this distribution (Equation 3).
3.3 Results and Discussion
Under laboratory conditions, a simulation of chemotaxis of 100 bacteria placed 6 cm away from a 5 mM malate source is shown in the movie below.
The distribution of malate in real soil is displayed in Figure 3. The paths of bacterial chemotaxis when placing bacteria at different positions in steady malate distribution is demonstrated in Figure 4.
Fig.3(a):Distribution of malate vs. distance (1D). Fig.3(b):Distribution of malate with radius (2D). Fig.3(b)shows the position of threshold detection concentration (1e-8 M at radius = 0.028 m) and the saturation concentration (1e-5 M at radius = 0.012).
Fig.4:Chemotaxis with placing bacteria at different starting positions. Blue: 2×105 seconds chemotaxis starts at radius = 0.015 m (in between 0.012m and 0.028m), green: 2×105 seconds chemotaxis starts at radius = 0.008 m, which is less than 0.012. This shows that the chemotactic response of bacteria is inefficient when they are placed between 0.0028 m and 0.0012 m from the root tip. The green line shows that the bacteria can be maintained close to the seed when it is placed at a distance less than 0.012 m from the root tip, therefore it is suggested for our project that the bacteria should be placed at a distance less than 0.012 m.
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[8] https://2009.igem.org/Team:Aberdeen_Scotland/chemotaxis
[9] Enrico Martinoia and Doris Rentsch. ‘Malate Compartmentation-Responses to a Complex Metabolism’ Annual Review of Plant Physiology and Plant Molecular Biology Vol. 45: 447-467 (Volume publication date June 1994) DOI: 10.1146/annurev.pp.45.060194.002311
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