Team:Tokyo Metropolitan/Notebook/S15
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Revision as of 10:25, 5 October 2011
-Targeting-
-Plasmid extraction and DNA refining
Extracted plasmids for targetingDevice and P0440.
Results
Targeting
75 ng/ul
P0440
20 ng/ul
-Electrophoresis
Checked for P0440, Targeting and P0440.
Results
Checked to cut for P0440 by treat restriction enzyme but length was 1000 bp (too long).
-Transformation
Transformed P0140 instead of P0440. After, incubated at 37 deg C.