Team:Tokyo Metropolitan/Notebook/S15

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Revision as of 10:25, 5 October 2011

-Targeting-

-Plasmid extraction and DNA refining

Extracted plasmids for targetingDevice and P0440.


Results

Targeting

75 ng/ul

P0440

20 ng/ul


-Electrophoresis

Checked for P0440, Targeting and P0440.


Results

Checked to cut for P0440 by treat restriction enzyme but length was 1000 bp (too long).


-Transformation

Transformed P0140 instead of P0440. After, incubated at 37 deg C.