Team:Nevada/Project
From 2011.igem.org
Strongtruong (Talk | contribs) (→Task 1) Engineer Synechocystis to overproduce the hexose sugars) |
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https://static.igem.org/mediawiki/2011/f/f0/AGP_INV_UNR1.jpg<br> | https://static.igem.org/mediawiki/2011/f/f0/AGP_INV_UNR1.jpg<br> | ||
+ | |||
+ | ''' | ||
+ | == Construct Components == | ||
+ | ''' <br> | ||
'''ADP Glucose Pyrophosphorylase (AGP) Gene:''' The slr1176 open reading frame was PCR amplified from total Synechocystis PCC6803 genomic DNA and subcloned into pSB1a3. Forward and reverse primers were designed with 5’ extensions containing iGEM prefix and suffix sequences.<br> | '''ADP Glucose Pyrophosphorylase (AGP) Gene:''' The slr1176 open reading frame was PCR amplified from total Synechocystis PCC6803 genomic DNA and subcloned into pSB1a3. Forward and reverse primers were designed with 5’ extensions containing iGEM prefix and suffix sequences.<br> |
Revision as of 17:08, 26 September 2011
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