Team:EPF-Lausanne/Our Project/Reporter Systems

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To set up and characterize our two reporter systems, we assembled several plasmids that are to be co-transformed in order to create the whole system. We also had to characterize the two different promoters we used to control RFP expression: an existing Ptet promoter and a new Plac promoter. It is important to know the intrinsic strength of the RFP promoters in order to be able to properly analyze the data generated by our two reporter systems.
To set up and characterize our two reporter systems, we assembled several plasmids that are to be co-transformed in order to create the whole system. We also had to characterize the two different promoters we used to control RFP expression: an existing Ptet promoter and a new Plac promoter. It is important to know the intrinsic strength of the RFP promoters in order to be able to properly analyze the data generated by our two reporter systems.
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Revision as of 03:48, 22 September 2011