Team:Paris Bettencourt/Data

From 2011.igem.org

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# '''SinOp reporter strain''', given by J.W. Veening, published in this paper [http://jb.asm.org/cgi/content/full/192/8/2053/DC1]
# '''SinOp reporter strain''', given by J.W. Veening, published in this paper [http://jb.asm.org/cgi/content/full/192/8/2053/DC1]
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<h2>The lambda switch system</h2>
<h2>The lambda switch system</h2>
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<h3>Data for our favorite new parts</h3>
<h3>Data for our favorite new parts</h3>
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# [http://partsregistry.org/Part:BBa_K606026 Main Page] - '''TetO Array, BBa_K606026''': 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
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# [http://partsregistry.org/Part:BBa_K606044 Main Page] - '''pVeg SpoVg CI TT, BBa_K606044''': Expression casette for for CI in B. subtilis.
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# [http://partsregistry.org/Part:BBa_K606003 Main Page] - '''Multihost replicative plasmid pHM3, BBa_K606003''': This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
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# [http://partsregistry.org/Part:BBa_K606025 Main Page] - '''YFP:tetR recombinant fusion protein, BBa_K606025''': This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).
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<h3>Data for Pre-existing Parts</h3>
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# [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - '''pVeg + SpoVG, BBa_K143053''' - (Imperial College, iGEM 2008)
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# [http://partsregistry.org/Part:BBa_B0015 Main Page] - '''Double standard terminator, BBa_B0015''' - Standard double terminator.
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# [http://partsregistry.org/Part:BBa_C0052 Main Page] - '''CI, BBa_B0052''' - CI gene from the lambda phage.
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<h2>GFP diffusion</h2>
<h2>GFP diffusion</h2>

Revision as of 02:51, 22 September 2011

Team IGEM Paris 2011

Data page

The YFP concentration system

The YFP concentration system principle

Data for our favorite new parts

  1. [http://partsregistry.org/Part:BBa_K606026 Main Page] - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.

Data for Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K090403:Experience Experience] - Gram positive chromosomal integration plasmid, BBa_K090403 (Cambridge, iGEM 2008):



The T7 polymerase diffusion system

The T7 polymerase diffusion system principle

Data for our favorite new parts

  1. [http://partsregistry.org/Part:BBa_K606026 Main Page] - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
  2. [http://partsregistry.org/Part:BBa_K606003 Main Page] - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
  3. [http://partsregistry.org/Part:BBa_K606025 Main Page] - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).

Data for Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):



The tRNA Amber diffusion system

The tRNA Amber diffusion system principle

Data for our favorite new parts

  1. [http://partsregistry.org/Part:BBa_K606026 Main Page] - TetO Array, BBa_K606026: 10kb of Tet operator sequence. YFP:tetR will bind to it and concentrate the fluorescence.
  2. [http://partsregistry.org/Part:BBa_K606003 Main Page] - Multihost replicative plasmid pHM3, BBa_K606003: This plasmid is an multihost plasmid, replicated both in E. Coli at high copy number (>200) and low copy in B. Subtilis (about 20).
  3. [http://partsregistry.org/Part:BBa_K606025 Main Page] - YFP:tetR recombinant fusion protein, BBa_K606025: This is composed by the Yellow Fluorescent Protein (YFP) and the Tetracycline Repressor Protein (tetR) and bind to the tet operator sequence (tetO).

Data for Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - pVeg + SpoVG, BBa_K143053 (Imperial College, iGEM 2008):



The ComS diffusion system

The ComS diffusion system principle

Data for our favorite new parts

  1. [http://partsregistry.org/Part:BBa_K606037 Main Page] - ComS gene, BBa_K606037: The comS gene can trigger the MecA/ComS/ComK system. The start and stop codon has been changed for more standard codons.
  2. [http://partsregistry.org/Part:BBa_K606038 Main Page] - ComS gene Codon optimized, BBa_K606038: This is the same gene than the previous one, but it has been synthetized de novo.

Data for Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K143053 Main Page] - pVeg + SpoVG, BBa_K143053 promoter and RBS for B. subtilis (Imperial College, iGEM 2008)
  2. [http://partsregistry.org/Part:BBa_B0015 Main Page] - Double standard terminator, BBa_B0015 standard double terminator
  3. [http://partsregistry.org/Part:BBa_K143021 Main Page] - RFP, BBa_K143021 RFP m-cherry.
  4. Elowitz's pComG-CFP/pComS-YFP reporter strain - Competence reporter strain. Published in this paper [http://www.elowitz.caltech.edu/publications/Tunability.pdf]
  5. CodY- strain - From Abraham L. Sonenshein's laboratory, Tufts university [http://www.tufts.edu/sackler//facultyIntros/sonensheinA.html]

The SinOp system

Data for our favorite new parts

  1. [http://partsregistry.org/Part:BBa_K606046 Main Page] - KinA gene, BBa_K606046 KinA gene. SinOp kinase.
  2. [http://partsregistry.org/Part:BBa_K606047 Main Page] - pVeg-SpoVg-KinA gene, BBa_K606047 KinA gene with B. subtilis promoter

Data for Pre-existing Parts

  1. SinOp reporter strain, given by J.W. Veening, published in this paper [http://jb.asm.org/cgi/content/full/192/8/2053/DC1]

The lambda switch system

The lambda switch system principle

Data for our favorite new parts

  1. [http://partsregistry.org/Part:BBa_K606044 Main Page] - pVeg SpoVg CI TT, BBa_K606044: Expression casette for for CI in B. subtilis.

Data for Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K143053:Experience Experience] - pVeg + SpoVG, BBa_K143053 - (Imperial College, iGEM 2008)
  2. [http://partsregistry.org/Part:BBa_B0015 Main Page] - Double standard terminator, BBa_B0015 - Standard double terminator.
  3. [http://partsregistry.org/Part:BBa_C0052 Main Page] - CI, BBa_B0052 - CI gene from the lambda phage.



GFP diffusion

Nanotube and GFP.jpeg

Data for Pre-existing Parts

  1. [http://partsregistry.org/Part:BBa_K143079 Experience] - Pveg-spoVG GFP expression construct, BBa_K143079 (Imperial College, iGEM 2008):