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Team:ETH Zurich/Achievements/Results
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| - | In general the test system for characterization of our BioBrick parts BBa_K625000respectively BBa_K625001 | + | In general the test system for characterization of our BioBrick parts BBa_K625000respectively BBa_K625001 worked as expected. In both system we can see that the fluorescence decreases with increasing amounts of anhydrotetracycline. This indicates that LacI<sub>M1</sub> is induced and thus represses the expression of ''gfp''. By addition of IPTG LacI<sub>M1</sub> gets inhibited and therefore the GFP response increses. This tendency can be observed both in figure 1 and figure 2. |
Revision as of 02:18, 22 September 2011
| Experimental Results |
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Results of BBa_K625000/BBa_K625001 characterization
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Figure 1: time course of fluorescence depending on different expression levels of the transcriptional regulator LacIM1. pSB3C5 was used as plasmid backbone for the test system.
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Figure 2: time course of fluorescence depending on different expression levels of the transcriptional regulator LacIM1. pSB1AK3 was used as plasmid backbone for the test system.
In general the test system for characterization of our BioBrick parts BBa_K625000respectively BBa_K625001 worked as expected. In both system we can see that the fluorescence decreases with increasing amounts of anhydrotetracycline. This indicates that LacIM1 is induced and thus represses the expression of gfp. By addition of IPTG LacIM1 gets inhibited and therefore the GFP response increses. This tendency can be observed both in figure 1 and figure 2.
