Team:Baltimore/Notebook

From 2011.igem.org

(Difference between revisions)
(August)
(September)
Line 69: Line 69:
'''Week 1:'''    [[Thursday, September 1]];  [[Friday, September 2]];  [[Saturday, September 3]];  [[Sunday, September 4]]; <br>
'''Week 1:'''    [[Thursday, September 1]];  [[Friday, September 2]];  [[Saturday, September 3]];  [[Sunday, September 4]]; <br>
'''Week 2:''' [[Tuesday, September 6]]; [[Thursday, September 8]]; [[Friday, September 9]];  [[Saturday, September 10]];  [[Sunday, September 11]];
'''Week 2:''' [[Tuesday, September 6]]; [[Thursday, September 8]]; [[Friday, September 9]];  [[Saturday, September 10]];  [[Sunday, September 11]];
 +
'''Week 3:''' [[Tuesday, September 13]]; [[Thursday, September 15]]; [[Friday, September 16]];  [[Saturday, September 17]];  [[Sunday, September 18]];

Revision as of 00:03, 9 September 2011

Home Team Project Parts Submitted to the Registry Modeling Notebook Solutions Safety Attributions Hardware Material Safety Data Sheets [http://partsregistry.org/assembly/libraries.cgi?id=35|MIT Parts Registry]

Contents

Notebook

For more information about the project click Dr. Tom's Notes

Run through for abstract and scheduling:

Start with plasmid with taq gene and pst1 site

Remove pst1 site from taq coding sequence

  • In order to remove the restriction site, do site directed mutagenesis (1day)
  • PCR
  • Digestion
  • Transformation
  • Screening (1day)
    • Dilute DNA
    • Colony PCR individually
    • Digestion of PCR product with pst1
    • Run gel ~2500bp

Add biobrick prefix/suffix to taq coding sequence

  • PCR and add the prefix and suffix as primers (one prefix has an extra AG-make sure to use the correct one) (1-2 days)
    • Run gel
    • Cut out of gel
  • Cut with restriction enzyme
  • Clean up DNA
  • Ligation with vector (could be vector with the terminator sequence, promoter and RBS)
  • Transformation

Add a promoter, transcriptional terminator, ribosome binding site (RBS)

  • Screen colonies (1 day)
    • Colony PCR
    • Restriction Digestion
    • Clean up DNA
  • Sequence (1 day)

Make taq protein

Compare it to other enzymes and make sure it works

Calendar


July

Week 1: Thursday, July 7;

Week 2: Tuesday, July 12; Wednesday, July 13; Thursday, July 14; Friday, July 15; Saturday, July 16; Sunday, July 17;

Week 3: Tuesday, July 19; Wednesday, July 20; Thursday, July 21; Friday, July 22; Saturday, July 23; Sunday, July 24;

Week 4: Tuesday, July 26; Wednesday, July 27; Thursday, July 28; Friday, July 29; Saturday, July 30; Sunday, July 31;

August

Week 1: Tuesday, August 2; Wednesday, August 3; Thursday, August 4; Friday, August 5; Saturday, August 6; Sunday, August 7;

Week 2: Week Off

Week 3: Tuesday, August 16; Wednesday, August 17; Thursday, August 18; Friday, August 19; Saturday, August 20; Sunday, August 21;

Beginning in Week 4, we will be tightening up our iGEM ship for a multitude of reasons. READ the New Rules.

Week 4: Tuesday, August 23; Wednesday, August 24; Thursday, August 25; Friday, August 26; Saturday, August 27; Sunday, August 28;

Week 5: Tuesday, August 30; Wednesday, August 31;

September

Week 1: Thursday, September 1; Friday, September 2; Saturday, September 3; Sunday, September 4;
Week 2: Tuesday, September 6; Thursday, September 8; Friday, September 9; Saturday, September 10; Sunday, September 11; Week 3: Tuesday, September 13; Thursday, September 15; Friday, September 16; Saturday, September 17; Sunday, September 18;