Team:EPF-Lausanne/Notebook/August2011

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(Difference between revisions)
(Wednesday, 17 August 2011)
(Wednesday, 17 August 2011)
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Alessandro proceeded with the day 3 of competent cells making protocol.
Alessandro proceeded with the day 3 of competent cells making protocol.
Alessandro ran the PCR to amplify T7lac2-RFP again and it' ok (the following day he will do the PCR purification) and PCR purified the pSB3K1 backbone (succesfully amplified the day before) as well as the T7-lysis again but this time the yeld was higher. Alessandro has made the Gibson assembly for pSB3C5 and pSB3K1 with T7-RFP and with T7-Lysis (the Gibosn for the T7lac2 hasn't been done because we ran out of Gibson master mix).
Alessandro ran the PCR to amplify T7lac2-RFP again and it' ok (the following day he will do the PCR purification) and PCR purified the pSB3K1 backbone (succesfully amplified the day before) as well as the T7-lysis again but this time the yeld was higher. Alessandro has made the Gibson assembly for pSB3C5 and pSB3K1 with T7-RFP and with T7-Lysis (the Gibosn for the T7lac2 hasn't been done because we ran out of Gibson master mix).
 +
Alessandro miniprepped pSB3K1.
Vincent double-checked his mutant results using CLUSTALW and the results are available for [[File:P39q_y42m_official_alignment_clustalw.txt‎| P39Q_Y42M ]]
Vincent double-checked his mutant results using CLUSTALW and the results are available for [[File:P39q_y42m_official_alignment_clustalw.txt‎| P39Q_Y42M ]]

Revision as of 15:22, 17 August 2011