Team:EPF-Lausanne/Tools/Microfluidics/HowTo2

From 2011.igem.org

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(A basic microfluidics control setup)
(Hooking it all up: preparing tubes and priming the chip)
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The presence of bubbles in a channel increases its fluidic resistance, and therefore perturbates or even blocks flow. To avoid this, the first step in running a chip is ''priming'': filling all the channels with fluid, then closing all the micro-valves and keeping pressure applied, until any air remaining in the chip diffuses through the chip walls and disappears.
The presence of bubbles in a channel increases its fluidic resistance, and therefore perturbates or even blocks flow. To avoid this, the first step in running a chip is ''priming'': filling all the channels with fluid, then closing all the micro-valves and keeping pressure applied, until any air remaining in the chip diffuses through the chip walls and disappears.
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Once all the channels are filled, the experiments can be ran. For the worm chip, we would prime it with buffer, then introduce the worm when the chip is filled. For the MITOMI chip, we would flow in proteins and DNA.
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Once all the channels are filled, the experiments can begin. For the worm chip, we would prime the device with buffer, then introduce the worm after the chip is filled. For the MITOMI chip, we would flow in various biomolecules (neutravidin, bovine serum albumin, antibodies, cell-free extracts) and DNA.
== Complete parts list ==
== Complete parts list ==

Revision as of 20:46, 21 September 2011