Latest revision as of 15:39, 5 October 2011

-Speeding Up-

Amplified HNS(wt),I20260 with PCR and purified these PCR products.

Plasmid extraction and purification to get J23100・I714051,but there was enough plasmid.

@plasmid extraction OriTr

@DNA refine OriTr

@DNA densitometry

@restriction emzyme(EcoRⅠ and SpeⅠ, ScoRⅠ and XbaⅠ) OriTr，OriTr+GFP

-DNA refining

P0440

no.1 + no.3 143

no.4 + no.5 130

J23100

no.1 + no.3 170

no.4 + no.5 208

K317037

no.3 + no.5 210

-Digestion

P0440xba+pst

J23100Spe+pst

K317037xba+pst

after digested, incubated for 5 hours at 37 deg C.

-Electrophoresis

Results

Only had cut K317037

-Gel extraction

Gel extracted for K317037, then DNA refined

-Mesurement of concentration

J23100 -> 90

K317037 no.2 102, no.4 75

(ng/ul)

@@ Line 8: / Line 8: @@
 = -Visualization- =
 Plasmid extraction and purification to get J23100・I714051,but there was enough plasmid.
+------------------------------------
+@plasmid extraction OriTr
+@DNA refine OriTr
+@DNA densitometry
+@restriction emzyme(EcoRⅠ and SpeⅠ, ScoRⅠ and XbaⅠ) OriTr，OriTr+GFP
+= -Targeting- =
+-DNA refining
+P0440
+no.1 + no.3 143
+no.4 + no.5 130
+J23100
+no.1 + no.3 170
+no.4 + no.5 208
+K317037
+no.3 + no.5 210
+-Digestion
+P0440xba+pst
+J23100Spe+pst
+K317037xba+pst
+after digested, incubated for 5 hours at 37 deg C.
+-Electrophoresis
+Results
+Only had cut K317037
+-Gel extraction
+Gel extracted for K317037, then DNA refined
+-Mesurement of concentration
+J23100 -> 90
+K317037 no.2 102, no.4 75
+(ng/ul)