Team:ZJU-China/Protocol

From 2011.igem.org

(Difference between revisions)
 
(One intermediate revision not shown)
Line 10: Line 10:
<style>
<style>
 +
#biofilmpro{width:770px;}
#p_intro{color:white;
#p_intro{color:white;
font-size:20px;
font-size:20px;
Line 57: Line 58:
   </tr>
   </tr>
</table>
</table>
-
<p style="margin-left:10px;">This group...........</p>
+
<p style="margin-left:10px;"></p>
</div>
</div>
Line 121: Line 122:
            
            
   <div class="bigblock">
   <div class="bigblock">
-
<div class="block" id="nsheet">  
+
<div class="block" id="biofilmpro">
-
<h3>Week1</h3><hr/>  
+
<h3>Biofilm Formation</h3>
-
+
<hr/>
-
<table id="notesheet" width="650" border="1" cellspacing="0" cellpadding="1">  
+
<p>Biofilm Formation Protocol with common lab equipment:</p>
-
  <tr><td width="76">Day</td><td width="349">Note</td></tr>  
+
<a style="display:block;margin:20px;" href="http://ung.igem.org/wiki/images/b/b1/Biofilm_protocol.pdf"><img src="http://ung.igem.org/wiki/images/1/1b/Adobe_Reader_Logo.jpg" width="40" height="40"/>download PDF version</a>
-
 
+
</div>
-
  <tr>  
+
 
-
    <td id="sheetleft">Jul.4th Monday</td>  
+
 
-
    <td><table id="intable" width="328" border="0" cellspacing="0" cellpadding="1">
+
<!-- 11111111111111111111111111111111111111111111111111 -->
-
 
+
<div class="block" id="summ">
-
    <td width="128">• Aerobic cultivation of DH5α</td>
+
<h3>Basic Protocols</h3><hr/>
-
 
+
<p>&nbsp;</p>
-
 
+
<div class="block" id="monday">
-
    <td width="196">• Preparation of apparatus for the formation of biofilm</td>  
+
<p>AxyPrep™ Plasmid Miniprep Kit</p>
-
 
+
<hr/>
-
</table></td>  
+
<a style="display:block;margin:20px;" href="http://static0.jadedpixel.com/s/files/1/0001/7488/files/axyprep_plasmid_mini_protocol.pdf"><img src="http://ung.igem.org/wiki/images/1/1b/Adobe_Reader_Logo.jpg" width="40" height="40"/>download PDF version</a>
-
  </tr>
+
</div>
-
  <tr>  
+
 
-
    <td id="sheetleft">Jul 5th Tuesday
+
<!-- 2222222222222222222222222222222222222222222 -->
-
</td>  
+
 
-
    <td>&nbsp;</td>  
+
<div class="block" id="monday">
-
  </tr>  
+
<p>AxyPrep™ PCR Cleanup Kit</p>
-
  <tr>
+
<hr/>
-
    <td id="sheetleft">Jul.6th
+
<a style="display:block;margin:20px;"  
-
Wednesday
+
href="http://static3.jadedpixel.com/s/files/1/0001/7488/files/axyprep_pcr_cleanup_protocol.pdf"><img src="http://ung.igem.org/wiki/images/1/1b/Adobe_Reader_Logo.jpg" width="40" height="40"/>download PDF version</a>
-
</td>  
+
</div>
-
    <td><table width="217" border="0" cellspacing="0" cellpadding="1">
+
 
-
  <tr>
+
<!-- 3333333333333333333333333333333333333 -->
-
    <td width="215">•Receiving  primers ordered previously</td>  
+
 
-
   
+
<div class="block" id="monday">
-
  </tr>  
+
<p>TaKaRa Agarose Gel DNA Purification Kit Ver.2.0</p>
-
</table>  
+
<hr/>
-
</td>
+
<a style="display:block;margin:20px;"  
-
  </tr>  
+
href="http://www.takara.com.cn/explain/D/DV805A.pdf"><img src="http://ung.igem.org/wiki/images/1/1b/Adobe_Reader_Logo.jpg" width="40" height="40"/>download PDF version</a>
-
  <tr>
+
</div>
-
    <td id="sheetleft">Jul.7th
+
 
-
Thursday
+
<!-- 444444444444444444444444444444444 -->
-
</td>  
+
 
-
    <td><table width="238" border="0" cellspacing="0" cellpadding="1">
+
<div class="block" id="monday">
-
  <tr>
+
<p>Chemically competent E.coli preparation.</p>
-
    <td width="200">• Preparation of the aliquot of the primers</td>
+
<hr/>
-
    <td width="200">• Something wrong with a shaking incubator</td>  
+
<a style="display:block;margin:20px;"  
-
  </tr>  
+
href="http://www.openwetware.org/wiki/Preparing_chemically_competent_cells">See official protocol</a>
-
</table>  
+
</div>
-
</td>
+
 
-
  </tr>  
+
<!-- 55555555555555555555555555555555555555 -->
-
  <tr>
+
 
-
    <td id="sheetleft">Jul.8th
+
<div class="block" id="monday">
-
Friday
+
<p>Transformation</p>
-
</td>  
+
<hr/>
-
    <td><table width="222" border="0" cellspacing="0" cellpadding="1">
+
<a style="display:block;margin:20px;"  
-
      <tr>
+
href="http://www.openwetware.org/wiki/Transforming_chemically_competent_cells_%28Inoue%29">See official protocol</a>
-
        <td width="155">• Preparation of culture plates for the transformations</td>
+
</div>
-
      </tr>
+
 
-
    </table></td>  
+
<!-- 666666666666666666666666666666666 -->
-
  </tr>  
+
 
-
  <tr>
+
<div class="block" id="monday">
-
    <td id="sheetleft">Jul.9th
+
<p>Colony PCR:</p>
-
Saturday
+
<hr/>
-
</td>
+
<a style="display:block;margin:20px;"  
-
    <td><table width="313" border="0" cellspacing="0" cellpadding="1">  
+
href="http://www.openwetware.org/wiki/Endy:Colony_PCR">See official protocol</a>
-
  <tr>  
+
</div>
-
    <td width="127">• Preparation of culture plates for the transformations</td>  
+
<div class="block" id="mondy">
-
    <td width="182">• protocols of transformation</td>
+
<p>Agarose gel electrophoresis</p><hr/>
-
  </tr>
+
<a style="display:block;margin:20px;"
-
</table>  
+
href="http://www.openwetware.org/wiki/Agarose_gel_electrophoresis">See official protocol</a>
-
</td>  
+
</div>
-
  </tr>  
+
</div>
-
  <tr>  
+
-
    <td id="sheetleft">Jul.10th
+
-
Sunday
+
-
</td>  
+
-
    <td><table width="246" border="0" cellspacing="0" cellpadding="1">  
+
-
      <tr>  
+
-
        <td>• Several colonies were picked up and cultivated in  5mL LB medium.
+
-
•Cryosectioning of biofilm
+
-
</td>  
+
-
      </tr>  
+
-
    </table></td>  
+
-
  </tr>  
+
-
+
-
</table>  
+
-
<p>&nbsp;</p>  
+
-
</div>  
+
<script type="text/javascript">
<script type="text/javascript">

Latest revision as of 03:01, 6 October 2011

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">

Protocol

Biofilm Formation

Biofilm Formation Protocol with common lab equipment:

download PDF version

Basic Protocols

 

AxyPrep™ Plasmid Miniprep Kit

download PDF version

AxyPrep™ PCR Cleanup Kit

download PDF version

TaKaRa Agarose Gel DNA Purification Kit Ver.2.0

download PDF version

Chemically competent E.coli preparation.

See official protocol

Transformation

See official protocol

Colony PCR:

See official protocol

Agarose gel electrophoresis

See official protocol

Retrieved from "http://2011.igem.org/Team:ZJU-China/Protocol"