Team:ZJU-China/Protocol

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Biofilm Formation Protocol with common lab equipment:

AxyPrep™ Plasmid Miniprep Kit

AxyPrep™ PCR Cleanup Kit

TaKaRa Agarose Gel DNA Purification Kit Ver.2.0

Chemically competent E.coli preparation.

Transformation

Colony PCR:

Agarose gel electrophoresis