Team:Freiburg/Notebook/6 September

From 2011.igem.org

(Difference between revisions)
(NAME OF YOUR EXPERIMENT)
 
(9 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:Freiburg/Templates/header}}
{{:Team:Freiburg/Templates/header}}
-
 
+
<html>
 +
<div id="notebook-page-header">
 +
<div id="notebook-back" width="100px" >
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook/5_September">Previous entry</a>
 +
</div>
 +
<div id="notebook-title">
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 6 September </a>
 +
</div>
 +
<div id="notebook-next">
 +
<a href="https://2011.igem.org/Team:Freiburg/Notebook/7_September">Next entry</a>
 +
</div>
 +
</div>
 +
</html>
==Commons==
==Commons==
Line 52: Line 64:
we realised that there must have been a mistake during [https://2011.igem.org/Team:Freiburg/Notebook/22_August quickchange PCR] of CcaS from 22.08. <br/>The primer annealed in a unspecific way and because of that a big part of about 380 bp is missing.
we realised that there must have been a mistake during [https://2011.igem.org/Team:Freiburg/Notebook/22_August quickchange PCR] of CcaS from 22.08. <br/>The primer annealed in a unspecific way and because of that a big part of about 380 bp is missing.
 +
 +
 +
===Testdigest of 3A-assembly from 3.09===
 +
 +
{| style="color:black; background-color:lightgrey;" cellpadding="10%" cellpadding="15%" cellspacing="0" border="1" Align="center"
 +
|[[File:FR2011 Testdigest 6.09.Jpg |400px|400px]]
 +
PCB is supposed to be the assembled gene for ho1 and pcyA. <br>
 +
The expected bands for the insert are missing. <br>
 +
ho1 is the gel-extracted PR-ho1+ terminator construct. <br>
 +
The one sample with three bands will be send for sequencing.
 +
|}
 +
 +
 +
{| style="color:black; background-color:lightgrey;" cellpadding="10%" cellpadding="15%" cellspacing="0" border="1" Align="center"
 +
|[[File:FR2011 06.09.Jpg |350px|350px]]
 +
|}
==<span style="color:blue;">blue light receptor</span>==
==<span style="color:blue;">blue light receptor</span>==
Line 142: Line 170:
PCR products were loaded onto a gel, but there were no bands execpt for not3.
PCR products were loaded onto a gel, but there were no bands execpt for not3.
-
 
-
==<span style="color:red;">red light receptor</span>==
 
==<span style="color:orange;">Lysis cassette</span>==
==<span style="color:orange;">Lysis cassette</span>==
Line 229: Line 255:
==<span style="color:grey;">Precipitator</span>==
==<span style="color:grey;">Precipitator</span>==
-
===NAME OF YOUR EXPERIMENT===
+
===Transformation===
 +
 
 +
 
 +
{| style="border-spacing:0;"
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Name: Rüdiger
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Date: 06.09. - 07.09
 +
 
 +
|-
 +
| colspan="2"  style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Continue from Date 05.09. Name Rüdiger
 +
 
 +
Experiment Ligation
 +
 
 +
|-
 +
| colspan="2"  style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Project Name: Precipitator
 +
 
 +
|}
 +
Procedure
 +
 
 +
 
 +
# take cells from -80°C freezer and put them on ice! (every eppi contains about 400 μl cells)
 +
# thaw cells on ice 20 minutes
 +
# pipette 50 μl cells and 2 μl DNA into eppi still on ice!
 +
# Incubate for 30 minutes on ice
 +
# Heat at 42°C for 60 sec
 +
# Incubate on ice for 5 minutes
 +
# Add 200 μl LB Broth
 +
# Incubate for 2 hours at 37°C (cells with lysis cassette at 30°C!!)
 +
# Plate 50 μl and 200μl on two different LB/Agar plates with appropriate antibiotic resistance
 +
 
 +
'''Documentation:'''
 +
 
 +
Why are you doing this experiment? Name of the sample? Where are they stored? Name the vector with inserts, antibiotika resistance etc.
 +
 
 +
 
 +
{| style="border-spacing:0;"
 +
| style="border:0.0069in solid #00000a;padding-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Picked colonies from plates 1 - 10
 +
 
 +
|}
 +
 
 +
 
-
'''Investigators: NAME'''
 
{{:Team:Freiburg/Templates/footer}}
{{:Team:Freiburg/Templates/footer}}

Latest revision as of 00:26, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!