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Team:Groningen/project characterisation mu
From 2011.igem.org
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==cI Autoinducing Loops== | ==cI Autoinducing Loops== | ||
The autoinducing loop driven by cI will function as our first memory unit. | The autoinducing loop driven by cI will function as our first memory unit. | ||
| - | We aimed to find a combination of RBS, transcription factor and degradation tag that enables us to toggle between the two steady states in a robust way. | + | We aimed to find a combination of RBS, transcription factor and degradation tag that enables us to toggle between the two steady states in a robust way. To estimate the rate in which single cells are activated by too much leakage of cI protein we meassured the fluorescence in uninduced cells with a flow cytometer. |
| - | + | ||
{{FooterGroningen2011}} | {{FooterGroningen2011}} | ||
Revision as of 21:01, 20 September 2011
Memory Units
cI Autoinducing Loops
The autoinducing loop driven by cI will function as our first memory unit. We aimed to find a combination of RBS, transcription factor and degradation tag that enables us to toggle between the two steady states in a robust way. To estimate the rate in which single cells are activated by too much leakage of cI protein we meassured the fluorescence in uninduced cells with a flow cytometer.
