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Team:Paris Liliane Bettencourt/Notebook/2011/09/14/
From 2011.igem.org
Contents |
Kevin
Bad news from sequencing
It happend that the QCM done to remove PstI site has failed. There is an insert of 89 bp instead.
So we can't have the YFP:tetR biobricked for the first Jamboree but the tetO array is OK.
The priority is to characterize tetO array before the wiki freeze.
Characterisation of tetO array
We have to do a double transformation with YFP:tetR Wild Type (pFX234) and tetO array biobricked (K606026). And after we'll do microscopy in E. coli double transformated with YFP:tetR WT and tetO array BB, E. coli with YFP:tetR WT only (already have), E. coli with tetO array BB only (already have), Subtilis with tetO array.
Double Transformation
Let's start from miniprep to do the double transformation.
