Team:Washington/Magnetosomes/Magnet Toolkit

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(About the Magnetosome Toolkit)
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Using standard synthetic biology protocols and the vectors we created in our Gibson Assembly Toolkit, our team created the '''"Magnetosome Toolkit"''' which contains many of the genes required for magnetosome formation. Providing this toolkit to the Parts Registry will help allow future iGEM teams to manipulate and further understand magnetosome formation to eventually synthesize magnets in multiple organisms.  
Using standard synthetic biology protocols and the vectors we created in our Gibson Assembly Toolkit, our team created the '''"Magnetosome Toolkit"''' which contains many of the genes required for magnetosome formation. Providing this toolkit to the Parts Registry will help allow future iGEM teams to manipulate and further understand magnetosome formation to eventually synthesize magnets in multiple organisms.  
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As previously noted, magnetosome formation within the host-organism, ''Magnetospirillium magneticum'', strain AMB-1, is a highly regulated step-wise process. As shown in diagram of stepwise magnetosome construction above, some genes encode proteins that form an invagination of the inner membrane, other genes which help align the magnetosomes into their characteristics chains, and others which regulate the biomineralization of magnetic particles. Our team chose to focus on genes specifically related to magnetosome scaffolding/alignment since they are the essential foundation for magnetosome development. In addition, the creation of a scaffold to which other genes localize is highly applicable to systems in synthetic biology. (for more information, please see our Future Directions page)
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As previously noted, magnetosome formation within the host-organism, ''Magnetospirillium magneticum'', strain AMB-1, is a highly regulated step-wise process. As shown in diagram of stepwise magnetosome construction above, some genes encode proteins that form an invagination of the inner membrane, other genes which help align the magnetosomes into their characteristics chains, and others which regulate the biomineralization of magnetic particles. Our team chose to focus on genes specifically related to magnetosome scaffolding/alignment since they are the essential foundation for magnetosome development. In addition, the creation of a scaffold to which other genes localize is highly applicable to systems in synthetic biology. (for more information, please see our [https://2011.igem.org/Team:Washington/Magnetosomes/Future Future Directions] page)
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Our genes of interest were mamK and mamI as they have functions related to localization of the magnetosome. Specifically, mamK is a bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain. mamK is also shown to localize the mamI, which is loss inhibits membrane formation.  
Our genes of interest were mamK and mamI as they have functions related to localization of the magnetosome. Specifically, mamK is a bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain. mamK is also shown to localize the mamI, which is loss inhibits membrane formation.  
(for other gene functions, please see the iGEM Toolkits parts submitted page)
(for other gene functions, please see the iGEM Toolkits parts submitted page)
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==='''Toolkit Contruction'''===
==='''Toolkit Contruction'''===
[[File:Washington Methode image.jpg|700px|center]]
[[File:Washington Methode image.jpg|700px|center]]

Revision as of 00:09, 28 September 2011


iGEM Toolkits: Magnetosomes


What are magnetosomes? Where do they come from?

Magnetotactic Bacteria (left) and Magnetosome chains (right)


Magnetotactic bacteria are prokaryotic organisms that possess the unique ability to align themselves along a magnetic field. This form of taxis is made possible by the formation of a magnetosome. Magnetosomes are small invaginations of the bacterial inner membrane that contain magnetite particles.

These particles range in size between 20 and several hundred nanometers and are aligned in one or several chains along the long axis of the bacteria. These particles act together to form a magnetic dipole across the bacteria, allowing it to sense the earth’s magnetic field. Magnetotactic bacteria are microaerophilic; therefore, magnetosomes are thought to help aid the organism in its search for the optimal oxygen level from a search in three dimensional space (in all directions) to a one dimensional space along a single path.



A Closer look at Magnetosome Formation

Diagram of stepwise magnetosome construction within AMB-1


The formation of the magnetosome organelle is a highly regulated, step-wise process requiring a cascade of essential genes. The process is generally hypothesized as four stages:

  1. Membrane invagination
  2. Acquiring minerals for magnetite formation
  3. Iron-oxidation and reduction
  4. Magnetite nucleation and morphology regulation.


Earlier gene products must be present for later gene products to be formed as shown in the diagram on the right[http://www.pnas.org/content/107/12/5593.full.pdf+html]:




What did the UW iGEM team do with Magnetotactic Bacteria?


It is thought that many of the essential genes associated with magnetosome formation are located within a well-conserved region known as the magnetosome island (MAI). The MAI consists of 14 gene clusters labeled R1-R14 (see diagram below).Our team focused on the genes of the mamAB gene cluster (R5), as they were previously shown to be the only cluster essential for magnetosome membrane biogenesis in AMB-1 (diagram show below).[http://www.pnas.org/content/107/12/5593/F1.expansion.html].

The goal of our project was to extract all the essential genes from (R5) required for magnetosome formation and express them in E. coli. We are doing this to learn more about magnetosome formation and the magnet synthesis mechanism, because many of the genes' functions are still unknown in the host species. Using the information we have gained, we have organized a Magnetosome Toolkit containing most of the essential genes for proper magnetosome formation. Ultimately, we would like to continue expanding the magnetosome toolkit to have enough parts to show complete magnetosome formation in E.coli.

Fig. 3: The mamAB operon (R5) located in the magnetosome island (MAI).


About the Magnetosome Toolkit


Using standard synthetic biology protocols and the vectors we created in our Gibson Assembly Toolkit, our team created the "Magnetosome Toolkit" which contains many of the genes required for magnetosome formation. Providing this toolkit to the Parts Registry will help allow future iGEM teams to manipulate and further understand magnetosome formation to eventually synthesize magnets in multiple organisms.

As previously noted, magnetosome formation within the host-organism, Magnetospirillium magneticum, strain AMB-1, is a highly regulated step-wise process. As shown in diagram of stepwise magnetosome construction above, some genes encode proteins that form an invagination of the inner membrane, other genes which help align the magnetosomes into their characteristics chains, and others which regulate the biomineralization of magnetic particles. Our team chose to focus on genes specifically related to magnetosome scaffolding/alignment since they are the essential foundation for magnetosome development. In addition, the creation of a scaffold to which other genes localize is highly applicable to systems in synthetic biology. (for more information, please see our Future Directions page)
Our genes of interest were mamK and mamI as they have functions related to localization of the magnetosome. Specifically, mamK is a bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain. mamK is also shown to localize the mamI, which is loss inhibits membrane formation. (for other gene functions, please see the iGEM Toolkits parts submitted page)

Toolkit Contruction

Washington Methode image.jpg


Please see our Result Summary page for constructions that were actually made.


References:

  1. Matsunaga, T., Okamura, Y., Fukuda, Y., Wahyudi, A.T., Murase, Y., Takeyama, H. (2005). Complete genome sequence of the facultative anaerobic Magnetotactic bacterium Magnetospirillum sp. strain AMB-1. DNA research; 12: 157-166. Doi:10.1093/dnares/dsi002.
  2. Murat, D., Quinlan, A., Vali, H., Komeili, A. (2010). Comprehensive genetic dissection of the magnetosome gene island reveals the step-wise assembly of a prokaryotic organelle. PNAS; 107 (12): 5593-5598. Doi:10.1073/pnas.0914439107.
  3. Murat, D., Quinlan, A., Vali, H., Komeili, A. (2010). Supporting Information. PNAS; 107 (12): 5593-5598. Doi:10.1073/pnas.0914439107.
  4. Quinlan, A., Murat, D., Vali, H., Komeili, A. (2011).The HtrA/DegP family protease MamE is a bifunctional protein with roles in magnetosome protein localization and magnetite biomineralization. Molecular Microbiology; 80 (4): 855-1131. Doi:10.1111/j.1365-2958.2011.07631.x.
  5. Richter, M., Kube, M., Bazylinski, D.A., Lombardot, T.,Glockner, F.O., Reinhardt, R., Shuler, D. (2007). Comparative genome analysis of four Magnetotactic bacteria reveals a complex set of group-specific genes implicated in magnetosome biomineralization and function. Journal of Bacteriology; 189(13): 4899-4910. Doi:10.1128/JB.00119-07.
  6. Rioux, J.B., Philippe, N., Pereia, S., Pignol, D., Wu, L.F., Ginet, N. (2010). A second actin-like mamK protein in Magnetospirillum magneticum AMB-1 encoded outside the genomic magnetosome island. PLoS ONE; 5(2): e9151. Doi:10.1371/journal.pone.0009151.