Team:Wageningen UR/Project/PartsProj1

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Building a Synchronized Oscillatory System

Synchroscillator: Data page/parts submitted to the registry

Parts Submitted to the Registry

[http://partsregistry.org/Part:BBa_K546003 BBa_K546003] - 3OC6HSL Receiver Device with lux pR: 3-oxohexanoyl-homoserine lactone (AHL) responsive element that produces a low constitutive level of luxR via lux pL. Combined with AHL, luxR activates lux pR.


[http://partsregistry.org/Part:BBa_K546002 BBa_K546002] - 3OC6HSL Receiver Device with a lux pR Regulated GFP (including a LVA Tag) Reporter: AHL responsive element that produces a low constitutive level of luxR via lux pL. Combined with AHL, luxR induces lux pR. lux pR regulates the expression of GFP. Upon addition of AHL the GFP expression increases.


[http://partsregistry.org/Part:BBa_K546000 BBa_K546000] - Autoinducer 3OC6HSL Receiver Device with a lux pR Controlled Autoinducer Synthetase: AHL responsive element that produces a low constitutive level of luxR via lux pL. Combined with AHL, luxR induces lux pR. lux pR regulates the expression of autoinducer synthetase.


[http://partsregistry.org/Part:BBa_K546005 BBa_K546005] - Autoinducer 3OC6HSL Receiver Device with a lux pR Regulated GFP (including a LVA Tag) & Autoinducer Synthetase: AHL responsive element that produces a low constitutive level of luxR via lux pL. Combined with AHL, luxR induces lux pR. lux pR regulates the expression of autoinducer synthetase. The system creates a positive feedback loop, yielding high titres of GFP.


[http://partsregistry.org/Part:BBa_K546001 BBa_K546001] - 3OC6HSL Receiver Device with a regulated 3OC6HSL inactivation enzyme gene: AHL responsive element that produces a low constitutive level of luxR via pL. Combined with 3-oxohexanoyl-homoserine lactone, luxR induces pR. lux pR regulates the autoinducer inactivation enzyme A (aiiA) gene that degrades AHL.