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Team:TU-Delft/Notebooks/Protocols/LBMedium
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Latest revision as of 12:51, 9 August 2011
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Protocol
LB Medium 1 liter
1. Measure 950 ml of demi water and put it in a flas
2. Add 10 grams of NaCl
3. Add 10 grams of Bacter Tryptone
4. Add 5 grams of Yeast extract
5. Bring the Ph to 7 in the "Kitchen" by pipettinga 10M NaOH
6. Fill up to 1 liter
7. Bring the flask to the autoclave
