Team:TU-Delft/Notebooks/Protocols/LBMedium

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Revision as of 13:07, 8 August 2011



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Protocol

LB Medium 1 liter

1. Measure 950 ml of demi water and put it in a flas

2. Add 10 grams of NaCl

3. Add 10 grams of Bacter Tryptone

4. Add 5 grams of Yeast extract

5. Bring the Ph to 7 in the "Kitchen" by pipettinga 10M NaOH

6. Fill up to 1 liter

7. Bring the flask to the autoclave