Team:HokkaidoU Japan/Protocols

From 2011.igem.org

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===Protein extraction from infected HeLa cells===
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===SDS-PAGE and Western Blot analysis===
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Revision as of 07:56, 29 September 2011

HokkaidoUJapan

Contents

Preparation of T3SS E. coli

Infection assay using onion cells

Infection assay using HeLa cells

Detection of injected protein using GSK tag

Protein extraction from infected HeLa cells

SDS-PAGE and Western Blot analysis


Hokkaido University COSMO BIO CO., LTD. Primary Cell Co.,Ltd. (有) メンデル工房 サイエンス教室