Team:Freiburg/Notebook/5 August
From 2011.igem.org
Contents |
Meeting
attendants: Jakob, Julia, Manuel, Rüdiger, Sandra, Theo, Tobi
time: 9:30 - 11:00
green light receptor
already done:
- CcaS: first quick change PCR removed the SpeI restriction site, EcoRI didn't work
- CcaR: first quick change PCR didn't work (EcoRI restriction site not removed), wrong PCR program
To-do:
- do the second quick change with the changed Ccas to remove the EcoRI restriction site
- repeat the quick change with the CcaR
- after the quick changes do a Gibson assembly with pcyA and ho1
- in parallel do 3A-assemblies to create the same construct as a backup plan
blue light receptor
already done:
- ordered new primers (the third pair)
- first primer pair had the overhang, second without overhang, third primer pair now without overhang and position shifted
To-do:
- try the gibson assembly again with the third primer pair
red light receptor
already done:
- 3A of pcyA and terminator sent for sequencing
- cph8 was sent for sequencing
- julia transformed the ompR promotor
To-do:
- add a Promotor-RBS to the pcyA-Terminator
- if the sequence is OK, amplify the part with the overhangs
- add terminator and Promotor-RBS
- do a miniprep of the ompR promotor
Lysis cassette
already done:
- 3A-assembly of the lysis casette
- 3A-assembly oth the GFP-PBD
- quick change PCR of the lysis casette (11 bases to insert the RBS)
To-do:
- do a miniprep from all three attempts
Precipitator
already done:
- waiting for godot
To-do:
- waiting for godot
other stuff
- start creating nice pictures to explain your subproject and write text to explain the pictures
- Sandra organized Freehand from the MPI, it is installed at one of the mac books
- give-aways: digital postcard, real postcard, fluorescent protein
- Tobi will ask at the financial department about the croud funding money
- Rüdiger will get us more media attention
- cooperation with other german teams: ask munich to do an appointment for a skype conforence about the light inducible promotors
- cooperation with Upsala: find an appointment for the skype talk
green light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
blue light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
red light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
NAME OF YOUR EXPERIMENT
Investigators: NAME
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