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Team:SJTU-BioX-Shanghai
From 2011.igem.org
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Merck Millipore
Shanghai Jiao Tong University
Bio-X Institutes
GenScript
SJTU-BioX-Shanghai iGEM team is designing a set of Codon-Switches that regulate target protein biosynthesis (translation).
In our Rare-Codon Switch, the translation of the protein can be finely turned up/down with the control of rare tRNA amount, aaRS that charges the rare tRNA and rare codons.
Besides, our device can be made into switches that can be turned on/off without background noise in two ways. One is to use stop codon as the controlling element, the Stop-Codon Switch. The other is to use any codon but the original start codon to initiate translation, the Initial-Codon Switch.
Our design has expanded the regulating tools for synthetic biology and introduced brand-new methods for protein function analysis. See more about project applications, click here.
Gold Medal and Best New BioBrick Part or Device, Engineered: BBa_K567011 & BBa_K567012 at Asia Jamboree. Advancing to World Champion!
Constructed three Codon Switches that controls protein biosynthesis, Rare-Codon Switch, Stop-Codon Switch and Initial-Codon Switch.
Used Rare-Codon Switch to turn up/down protein biosynthesis by controlling rare tRNA amount, aaRS and the number of rare codons.
Built our Rare-Codon Switch in silic and demonstrated the reliability of the model through our experiment results.
