Team:Minnesota/leex4462-08232011000000-109

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Ligation of pBAD+Silicatein and Digest of TOPO Silicatein

Project: Surface Display

Date: Tue, 23 Aug 2011 00:00:00 GMT

Author: Sukdong Lee (leex4462)

Access: Public

Revision History:

  • Mon, 29 Aug 2011 13:27:09 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)
  • Mon, 29 Aug 2011 13:29:56 GMT (leex4462): entry data updated by leex4462 (id=3)
  • Mon, 29 Aug 2011 13:30:55 GMT (leex4462): entry data updated by leex4462 (id=3)

Purpose:

-Attempt pBADD ligation in case the silicatein is toxic to the E. coli.

-Digest more silicatein since low concentrations


Procedures:

Same, used pBAD from an instructor





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