Team:Washington/Magnetosomes/GibsonResults

From 2011.igem.org

Comparison between pGA and pSB vectors

To quantitatively illustrate that better cloning efficiency is achieved by using pGA vectors vs standard pSB vectors, a pGA vector assay was conducted. The Gibson products made using our pGA vectors and the standard pSB vectors were plated separately over 6 plates, and the Gibson efficiency was calculated for each plate.

Efficiency was calculated by dividing the # of bright colonies by the # of total colonies - # of background colonies. Gibson Efficiency = Bright colonies/Total - Background)

pGA vector plate

pSB vector plate

Once the Gibson efficiences were calculated for each set of plates, the average Gibson efficiency was calculated. The average Gibson efficiency of using our pGA vectors was 0.991129032. This value is significantly higher than that of the pSB vectors (efficiency = 0.116395664).

Gibson Assembly Efficiency Comparison

Based on the comparison presented, we can be certain that our Gibson Assembly vectors (pGA vectors) are a great choice for gene assemblies!