Team:UCL London/Research/Stresslights/Experiments

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Modifying Oximeter in the lab

Aim

To modify and improve the oximeter device (BBa_K239011) using modified nark promoter (BBa_K239006) submitted by 2009 UCL iGEM team. Changes included replacing the normal GFP (green fluorescent protein) with an enhanced YFP (yellow fluorescent protein), which has an LVA tag added to its C terminal.

Ucl-content-Oximieter-circuit.jpg

Parts used

Part Name

Parts  ID

Parts Description

Length (bp)

Plasmid Backbone

mNARK promoter

BBa_K239006

promoter sensitive to hypoxia

89

on pUC57 (2710 bp) - ampicillin resistance

RBS

BBa_B0034

ribosome binding site

12

on pSB1A2 (2079 bp) - ampicillin resistance

YFP.LVA

BBa_E0032

enhanced yellow fluorescent protein with LVA tag

759

on pSB1A2 (2079 bp)

Terminator

BBa_B0015

double terminator (B0010-B0012)

129

on pSB1A2 (2079 bp)

pSB1K3

n/a

standard plasmid backbone (kanamycin resistance)

2204

n/a

pSB1T3

n/a

standard plasmid backbone (tetracycline resistance)

2463

n/a

pSB1C3

n/a

standard plasmid backbone (chloramphenicol resistance)

2070

n/a

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