Team:UCL London/Research/Supercoilometer/Experiments

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Building a Supercoilometer in the lab

Aim

To prepare a BioBrick from the tyrT promoter cloned from the E. coli genome and then construct and characterise a device which can detect the level of negative supercoiling in a closed circular plasmid.

Parts used

Part Name

Parts  ID

Parts Description

Length (bp)

Plasmid Backbone

RBS+CFP.LVA+Terminator

BBa_E0422

RBS + cyan fluorescent protein with LVA tag + terminator

917

on pSB1A2 plasmid (2079 bp) - ampicillin resistance

pSB1C3

n/a

standard plasmid backbone (chloramphenicol resistance)

2070

n/a
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