Team:Washington/Protocols
From 2011.igem.org
General Agarose Gel Electrophoresis
General Transformation Protocol
[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of how Kunkel Mutagensis works]
Standard 1L Expression Purification
Make It: Diesel Production Protocols
Alkane Biosynthesis media and extraction
Cell Lysate Assay by Decarbonylase Redesign Team
Break It: Gluten Destruction Protocols
Small Scale (50mL) Protein Expression and Purification
Make It: iGem Toolkits
Electroporation (Transformation)
High-Yield PCR (Full-Gene Assembly)
Isolation of Plasmid DNA (miniprep)
Induction Studies of Proteins Fusions (mam-sfGFP)
Preparation of Overnight Cultures
Wiki Design
Wiki Design Tools (Wiki Markup, WikiDust, etc.)