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Contents 1 HokkaidoU Japan 2 Primers 2.1 Note 2.2 General Primers 2.3 Primers Used for Backbone Construction 2.4 Sequencing Primer

General Protocols Infection assay

Primers

Note

Primer Name	Whole Sequence
	F/R	Annealing Sequence	Tm	Adding Sequence

General Primers

EX_F	gcagaattcgcggccgcttctagag
	Forward	Biobrick Prefix	74.5 C	None
PS_R	agcctgcagcggccgctactagta
	Reverse	Biobrick Suffix	74.6 C	None
suffix_F	tactagtagcggccgctgcaggct
	Forward	Biobrick Suffix	74.6 C	None
prefix_R	ctctagaagcggccgcgaattctgc
	Reverse	Biobrick Prefix	74.5 C	None
100up_EX_F	aacctataaaaataccgcatacac
	Forward	100bp upstream from Biobrick prefix	62.7 C	None
200dn_PS_R	tcccctgattctgtggataaccgt
	Reverse	200bp downstream from Biobrick suffix	66.6 C	None

• [http://partsregistry.org/wiki/index.php?title=Part:BBa_K496000 See details of 100up_EX_F/200dn_PS_R] (partsregistry)

Primers Used for Backbone Construction

EX_RBS_SlrP_F	GCAGAATTCGCGGCCGCTTCTAGAaaagaggagaaaatatgtttaatattactaatatacaatctacggc
	Forward	RBS sequence, 5' terminal of SlrP coding sequence	69.7 C	Biobrick Prefix
PS_SlrP_R	AGCCTGCAGCGGCCGCTACTAGTggtaagtcctaatattttcagacgaag
	Reverse	3'terminal of SlrP coding sequence	64.5 C	Biofusion Suffix
Bsa1_dt_F	GGCGACTAGAGAGACCccaggcatcaaataaaacgaaag
	Forward	5'terminal of double terminator sequence	63.6 C	BsaI recognition site and its cleavage site
Bsa1_SlrP_R	GGCCTGGCCTGAGACCCCggtaagtcctaatattttcagacga
	Reverse	3'terminal of SlrP coding Sequence	63.0 C	BsaI recognition site and its cleavage site
Bsa1_GSK_SlrP_R	GGCCTGGCCTGAGACCCCACTTTCAGCGAAACTTGTAGTGCGAGGGCGACCACTCATggtaagtcctaatattttcagacga
	Reverse	3'terminal of SlrP coding Sequence	63.6 C	GSK Tag, BsaI recognition site and its cleavage site

• See details of BsaI Backbone (Project Page)

Sequencing Primer

SlrP_373_to_394_F	gaaagtcagtcacctatacccg
	Forward	SlrP coding sequence, from 373bp to 394 bp	63.4 C	None