Team:Tsinghua/Notebook/7 July 2011

From 2011.igem.org

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[[pET15b OmpA-GFP construct]]
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==[[pET15b OmpA-GFP construct]]==
   Colony PCR
   Colony PCR
   Tip a tiny bit of the colonies, dip some on a new LB plate and dive the others into PCR reaction mix.
   Tip a tiny bit of the colonies, dip some on a new LB plate and dive the others into PCR reaction mix.

Revision as of 14:41, 2 September 2011

pET15b OmpA-GFP construct

 Colony PCR
 Tip a tiny bit of the colonies, dip some on a new LB plate and dive the others into PCR reaction mix.
 Chose 20 clonies, 13 are correct.
 Inoculate 5 of the correct ones in 5ml LB Amp+
 -YX