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Team:Tsinghua/Notebook/7 July 2011
From 2011.igem.org
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| - | [[pET15b OmpA-GFP construct]] | + | ==[[pET15b OmpA-GFP construct]]== |
Colony PCR | Colony PCR | ||
Tip a tiny bit of the colonies, dip some on a new LB plate and dive the others into PCR reaction mix. | Tip a tiny bit of the colonies, dip some on a new LB plate and dive the others into PCR reaction mix. | ||
Revision as of 14:41, 2 September 2011
pET15b OmpA-GFP construct
Colony PCR Tip a tiny bit of the colonies, dip some on a new LB plate and dive the others into PCR reaction mix. Chose 20 clonies, 13 are correct. Inoculate 5 of the correct ones in 5ml LB Amp+ -YX
