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Team:Tsinghua/Notebook/4 July 2011
From 2011.igem.org
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| - | [[pET15b OmpA-GFP construct]] | + | ==[[pET15b OmpA-GFP construct]]== |
Digest pET15b vector with NdeI + BamHI (Takara), 37 centigrade, 3 hours. (Approx. 1ug) | Digest pET15b vector with NdeI + BamHI (Takara), 37 centigrade, 3 hours. (Approx. 1ug) | ||
Recycled digested vector, digested GFP fragment, digested OmpA fragment (previous day) via gel-extraction. | Recycled digested vector, digested GFP fragment, digested OmpA fragment (previous day) via gel-extraction. | ||
Revision as of 14:40, 2 September 2011
pET15b OmpA-GFP construct
Digest pET15b vector with NdeI + BamHI (Takara), 37 centigrade, 3 hours. (Approx. 1ug) Recycled digested vector, digested GFP fragment, digested OmpA fragment (previous day) via gel-extraction. Ligation 1ul vector + 10ul GFP + 10ul Takara Solution I. RT, 1h Transform DH5a competent cells, spread on Amp+ LB plates. YX
