"
Team:Tsinghua/Notebook/3 July 2011
From 2011.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | [[pET15b OmpA-GFP construct]] | + | ==[[pET15b OmpA-GFP construct]]== |
PCR GFP fragment flanked by NdeI + BamHI from peGFP-N1 vector. | PCR GFP fragment flanked by NdeI + BamHI from peGFP-N1 vector. | ||
Digest with NdeI, BamHI (Takara) 5 hours. | Digest with NdeI, BamHI (Takara) 5 hours. | ||
Latest revision as of 14:40, 2 September 2011
pET15b OmpA-GFP construct
PCR GFP fragment flanked by NdeI + BamHI from peGFP-N1 vector. Digest with NdeI, BamHI (Takara) 5 hours. PCR OmpA 540bp fragment from BL21 genome. Digest with NcoI (NEB) 3 hours. -YX
