Team:Tsinghua/Notebook/4 July 2011

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(Difference between revisions)
(Created page with "Digest pET15b vector with NdeI + BamHI (Takara), 37 centigrade, 3 hours. (Approx. 1ug) Recycled digested vector, digested GFP fragment, digested OmpA fragment (previous day) via...")
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Digest pET15b vector with NdeI + BamHI (Takara), 37 centigrade, 3 hours. (Approx. 1ug)
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[[pET15b OmpA-GFP construct]]
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  Digest pET15b vector with NdeI + BamHI (Takara), 37 centigrade, 3 hours. (Approx. 1ug)
-
Recycled digested vector, digested GFP fragment, digested OmpA fragment (previous day) via gel-extraction.
+
  Recycled digested vector, digested GFP fragment, digested OmpA fragment (previous day) via gel-extraction.
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  Ligation
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  1ul vector + 10ul GFP + 10ul Takara Solution I. RT, 1h
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Ligation
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  Transform DH5a competent cells, spread on Amp+ LB plates.
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+
  YX
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1ul vector + 10ul GFP + 10ul Takara Solution I
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-
 
+
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RT, 1h
+
-
 
+
-
Transform DH5a competent cells, spread on Amp+ LB plates.
+
-
 
+
-
YX
+

Revision as of 14:09, 2 September 2011

pET15b OmpA-GFP construct 

 Digest pET15b vector with NdeI + BamHI (Takara), 37 centigrade, 3 hours. (Approx. 1ug)
 Recycled digested vector, digested GFP fragment, digested OmpA fragment (previous day) via gel-extraction.
 Ligation
 1ul vector + 10ul GFP + 10ul Takara Solution I. RT, 1h
 Transform DH5a competent cells, spread on Amp+ LB plates.
 YX
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