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Team:Tsinghua/Notebook/3 July 2011
From 2011.igem.org
(Difference between revisions)
(Created page with "PCR GFP fragment flanked by NdeI + BamHI from peGFP-N1 vector. Digest with NdeI, BamHI (Takara) 5 hours. PCR OmpA 540bp fragment from BL21 genome. Digest with NcoI (NEB) 3 ho...") |
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| - | PCR GFP fragment flanked by NdeI + BamHI from peGFP-N1 vector. | + | [[pET15b OmpA-GFP construct]] |
| - | + | PCR GFP fragment flanked by NdeI + BamHI from peGFP-N1 vector. | |
| - | Digest with NdeI, BamHI (Takara) 5 hours. | + | Digest with NdeI, BamHI (Takara) 5 hours. |
| - | + | PCR OmpA 540bp fragment from BL21 genome. | |
| - | + | Digest with NcoI (NEB) 3 hours. | |
| - | PCR OmpA 540bp fragment from BL21 genome. | + | -YX |
| - | + | ||
| - | Digest with NcoI (NEB) 3 hours. | + | |
| - | + | ||
| - | -YX | + | |
Revision as of 14:08, 2 September 2011
PCR GFP fragment flanked by NdeI + BamHI from peGFP-N1 vector. Digest with NdeI, BamHI (Takara) 5 hours. PCR OmpA 540bp fragment from BL21 genome. Digest with NcoI (NEB) 3 hours. -YX
