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Team:Freiburg/Notebook/5 August
From 2011.igem.org
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==Meeting== | ==Meeting== | ||
| - | attendants: <br/> | + | attendants: Jakob, Julia, Manuel, Rüdiger, Sandra, Theo, Tobi <br/> |
| - | + | time: 9:30 - 11:00 | |
===<span style="color:green;">green light receptor</span>=== | ===<span style="color:green;">green light receptor</span>=== | ||
already done: | already done: | ||
| - | + | *CcaS: first quick change PCR removed the SpeI restriction site, EcoRI didn't work | |
| + | *CcaR: first quick change PCR didn't work (EcoRI restriction site not removed), wrong PCR program | ||
To-do: | To-do: | ||
| + | *do the second quick change with the changed Ccas to remove the EcoRI restriction site | ||
| + | *repeat the quick change with the CcaR | ||
| + | *after the quick changes do a Gibson assembly with pcyA and ho1 | ||
| + | *in parallel do 3A-assemblies to create the same construct as a backup plan | ||
===<span style="color:blue;">blue light receptor</span>=== | ===<span style="color:blue;">blue light receptor</span>=== | ||
| - | |||
already done: | already done: | ||
| - | + | *ordered new primers (the third pair) | |
| + | *first primer pair had the overhang, second without overhang, third primer pair now without overhang and position shifted | ||
To-do: | To-do: | ||
| + | *try the gibson assembly again with the third primer pair | ||
===<span style="color:red;">red light receptor</span>=== | ===<span style="color:red;">red light receptor</span>=== | ||
already done: | already done: | ||
| - | + | *3A of pcyA and terminator sent for sequencing | |
| + | *cph8 was sent for sequencing | ||
| + | *julia transformed the ompR promotor | ||
To-do: | To-do: | ||
| + | *add a Promotor-RBS to the pcyA-Terminator | ||
| + | *if the sequence is OK, amplify the part with the overhangs | ||
| + | *add terminator and Promotor-RBS | ||
| + | *do a miniprep of the ompR promotor | ||
===<span style="color:orange;">Lysis cassette</span>=== | ===<span style="color:orange;">Lysis cassette</span>=== | ||
already done: | already done: | ||
| - | + | *3A-assembly of the lysis casette | |
| + | *3A-assembly oth the GFP-PBD | ||
| + | *quick change PCR of the lysis casette (11 bases to insert the RBS) | ||
To-do: | To-do: | ||
| - | + | *do a miniprep from all three attempts | |
===<span style="color:grey;">Precipitator</span>=== | ===<span style="color:grey;">Precipitator</span>=== | ||
already done: | already done: | ||
| - | + | *waiting for godot | |
To-do: | To-do: | ||
| + | *waiting for godot | ||
| + | |||
| + | ===other stuff=== | ||
| + | *start creating nice pictures to explain your subproject and write text to explain the pictures | ||
| + | *Sandra | ||
| + | |||
<br/> | <br/> | ||
<br/> | <br/> | ||
Revision as of 09:27, 5 August 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
