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Team:Tsinghua/Notebook/9 May 2011
From 2011.igem.org
(Difference between revisions)
(Created page with "Overlapping PCR, get OmpA-SH3 fusion sequence. Digest with NcoI + BamHI Ligate into pET15b vector Transform Top10 Competent Cells.") |
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Overlapping PCR, get OmpA-SH3 fusion sequence. | Overlapping PCR, get OmpA-SH3 fusion sequence. | ||
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Digest with NcoI + BamHI | Digest with NcoI + BamHI | ||
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Ligate into pET15b vector | Ligate into pET15b vector | ||
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Transform Top10 Competent Cells. | Transform Top10 Competent Cells. | ||
Latest revision as of 14:45, 17 May 2011
Overlapping PCR, get OmpA-SH3 fusion sequence.
Digest with NcoI + BamHI
Ligate into pET15b vector
Transform Top10 Competent Cells.
