Team:Freiburg/Notebook/29 July

From 2011.igem.org

(Difference between revisions)
Line 22: Line 22:
already done:  
already done:  
-
*PCR of Lov-tap and Not-gate. Not-gate sequencing was good. PCR of Lov-tap did not work.
+
*PCR of Lov-tap and Not-gate. Not-gate sequencing was good. PCR of Lov-tap did not work the first time and the second time with the temperature gradient PCR
-
To-do: new Primer for Lov-tap.
+
To-do:  
 +
*creating new primer for the Lov-tap as the old ones had a high probabylity for secondary structures
===<span style="color:red;">red light receptor</span>===
===<span style="color:red;">red light receptor</span>===
already done:
already done:
-
 
+
*waiting for cph8 from Mexico
 +
*for pcyA we did a new transformation from the original plasmid,

Revision as of 09:43, 29 July 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

Meeting

attendants: Julia, Manuel, Rüdiger, Sandra, Theo, Tobi Time: 9:00 - 11:00

green light receptor

already done:

  • Ccas has to sites where we need to do a mutagenesis (quick change), both were done seperately, but the no colonies showed up (wrong PCR program)
  • CcaR has one site where we need to do a mutagenesis (quick change), this has been done, colonies were growing


To-do:

  • miniprep, digestion and sequencing of the CcaR clones
  • do the Ccas PCR again
  • next steps would be the big assembly wiht pcyA

blue light receptor

already done:

  • PCR of Lov-tap and Not-gate. Not-gate sequencing was good. PCR of Lov-tap did not work the first time and the second time with the temperature gradient PCR


To-do:

  • creating new primer for the Lov-tap as the old ones had a high probabylity for secondary structures

red light receptor

already done:

  • waiting for cph8 from Mexico
  • for pcyA we did a new transformation from the original plasmid,


To-do:

Lysis cassette

already done:


To-do:


Precipitator

already done:


To-do:

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

Glycerol stocks of Not-Gate

Investigators: Sandra

M45d was sequenced and we did glycerol stocks of bacteria containing M45d.

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

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