Meeting
attendants: Jakob, Julia, Manuel, Ruediger, Sandra, Sophie, Theo (later), Tobi
time: 9:00 - 10:30
green light receptor
already done:
- CcaS was amplified again from the genomic DNA
To-do:
- 3-A-Assembly of CcaR into an empty vector with a medium promotor
- 3-A-Assembly of CcaS into an empty vector with a weak promotor
- 3-A-Assembly of CcaR and CcaS to get the final construct
blue light receptor
already done:
- Primer-Design for the Gibson-Assembly
- growth medium without tryptophane is already ordered
To-do:
red light receptor
already done:
- one positive colony from the 3-A of ho1 and the terminator
- no positive colony from the 3-A of pcyA and the terminator
- the PCR to amplify cph8 didn't give a product, we asked the team from Mexico to send us the cph8
- the team from Upsala (Sweden) has succeeded in amplifying the cph8 (according to their notebook)
To-do:
- 3-A-Assembly of the ho1-term with a mediumPromotor-mediumRBS
Lysis cassette
already done:
- the quick change didn't work the first time, we should repeat this
- possible alternative to express the lysis cassette could be: temperature regulated RNA
To-do:
Precipitator
already done:
- the GFP-PBD was cloned (colonies showed up)
To-do:
- test digest and sequencing of the GFP-PBD (first have a look if you see the GFP fluorescence)
- possible test of the PBD: grow the E. coli, lyse them, put the lysate into a plate (96 well?), wash the wells to get rid of the other proteins, measure the GFP fluorescence with a plate reader
- microscope the E. coli cells
other suff
- think about possible give-aways for the sponsoring video
- jacob will create a Youtube account and upload the sponsoring video
- meeting for the wiki takeover: wednesday 9:00 am (new time: 12:15)
- modelling should be started now, either Ni-binding and release or GFP-PBD binding and release, Ruediger will do this
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