User:Lytao

From 2011.igem.org

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  <td width="350" rowspan="4" valign="top"></html>[[File:MODULE.png|300px|link=https://2011.igem.org/Team:USTC-China/Project/module]]<br/><br/>[[File:RESULTS.png|300px|link=https://2011.igem.org/Team:USTC-China/Project/results]]<br/><br/>[[File:GALLARY.png|300px|link=https://2011.igem.org/Team:USTC-China/Project/application]]<br/><br/>[[File:MODELING.png|302px|link=https://2011.igem.org/Team:USTC-China/Project/modeling]]<html>
 
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<h1>Sponsors</h1>
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<html lang="en">  
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<p align="center"> <a href="http://www.ustcif.org"><img src="https://static.igem.org/mediawiki/2011/b/ba/USTC_Software_IF_LOGO.jpg" width="306" height="59"></a></p>
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  <p align="left"><a href="http://www.teach.ustc.edu.cn/"><img src="https://static.igem.org/mediawiki/2011/1/11/USTC_Software_TAO_LOGO.png" width="306" height="56"></a></p>
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  <p align="left"><a href="http://biox.ustc.edu.cn/"><img src="https://static.igem.org/mediawiki/2011/d/d3/USTC_Software_sols.png" width="306" height="56"></a></p>
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  <p align="left"> <a href="http://en.gradschool.ustc.edu.cn/"><img src="https://static.igem.org/mediawiki/2011/e/ef/USTC_Software_ADGE_LOGO.png" width="306" height="56"></a></p>
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  <p align="left"><a href="http://sist.ustc.edu.cn/"><img src="https://static.igem.org/mediawiki/2011/1/18/USTC_Software_information.png" width="306" height="56"></a></p>
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  <p align="left"> <a href="#"><img src="https://static.igem.org/mediawiki/2011/2/25/USTC_Software_chemistry.jpg" width="306" height="56"></a></p>
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  <p align="left"><a href="http://math.ustc.edu.cn/Ch/"><img src="https://static.igem.org/mediawiki/2011/c/cf/USTC_Software_math.jpg" width="306" height="56"></a></p>
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<h1>Visitors</h1>
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<div id="clustrmaps-widget"></div><script type="text/javascript">var _clustrmaps = {'url' : 'https://2011.igem.org/Team:USTC-China', 'user' : 939206, 'server' : '4', 'id' : 'clustrmaps-widget', 'version' : 1, 'date' : '2011-10-05', 'lang' : 'zh', 'corners' : 'square' };(function (){ var s = document.createElement('script'); s.type = 'text/javascript'; s.async = true; s.src = 'http://www4.clustrmaps.com/counter/map.js'; var x = document.getElementsByTagName('script')[0]; x.parentNode.insertBefore(s, x);})();</script><noscript><a href="http://www4.clustrmaps.com/user/e40e54c6"><img src="http://www4.clustrmaps.com/stats/maps-no_clusters/2011.igem.org-Team-USTC-China-thumb.jpg" alt="Locations of visitors to this page" /></a></noscript>
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<h1>IGEM Main Page</h1><a href="https://2011.igem.org"><img src="https://static.igem.org/mediawiki/2011/7/70/Shell_res_doc_1_1_2_1.jpg" width="150"/></a>
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                    <div class="title_icon"><img src="https://static.igem.org/mediawiki/2011/0/07/USTC-China_checked.gif" alt="" title="" /></div>
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                        <h2> <span class="dark_blue">Project</span></h2>
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                      </html> [[File:USTC!1.png|170px|center]] <html>We have successfully constructed a novel system in which bacterial colonies will automatically divide into two parts after certain time. We have successfully assembled riboswitches finely tuned by small molecules and toggle switches pushed on and off while memorizing current state.</div>
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                        <a href="https://2011.igem.org/Team:USTC-China/Project" class="details">+ details</a>
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                    </div>
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}
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/* end twitter */
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    font-family:"Comic Sans MS", cursive;
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    padding: 5px 15px;
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    background-color:transparent;
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}
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/* TEAM PAGE */
 +
.bio {
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</head>
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</html>
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<html>
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<head>
 +
<!-- TODO: add pre/n buttons -->
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<script>
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$(document).ready(function(){
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  $('.bio').css('display','none');
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  $('.col_list li').css('cursor','pointer');
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  $('.col_list li').hover(function() {
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    $(this).css('background-color','#91A3ED');
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  $('.col_list li').click(function () {
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    $('#'+name+'bio').fadeIn(500);
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</script>
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</head>
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<body>
 +
<div id="col_nav">
 +
    <div class="col_list">
 +
        <h2>Weeks</h2>
 +
<ul>
 +
    <li id="yhl">Jun 28-July 2</li>
 +
    <li id="swl">July 3-July 9</li>
 +
    <li id="zlc">July 10-July 16</li>
 +
    <li id="fzz">July 17-July 23</li>
 +
    <li id="shl">July 24-July 30</li>
 +
    <li id="ml">July 31-Aug 6</li>
 +
    <li id="mps">Aug 7-Aug 13</li>
 +
            <li id="yfl">Aug 14-Aug 20</li>
 +
    <li id="dz">Aug 21-Aug 27</li>
 +
    <li id="lna">Aug 28-Sep 3</li>
 +
    <li id="jhp">Sep 4-Sep 10</li>
 +
    <li id="xlz">Sep 11-Sep 17</li>
 +
            <li id="wyy">Sep 18-Sep 24</il>
 +
    <li id="ytl">Sep 25-Oct 1</li>
 +
            <li id="nw">Oct 2-Oct 8</li>
 +
            <li id="yz">Oct 9-Oct 15</il>
 +
         
 +
</ul>
 +
    </div><!-- end undergrads -->
 +
</div>
 +
 +
 +
 
 +
<div id="col_left">
 +
 +
<div class="bio" id="yhlbio">
 +
<h1>Jun 28-July 2</h1>
 +
</html>
 +
2011.6.28
 +
----
 +
cultivate the bacteria of [http://partsregistry.org/Part:BBa_K557000 cheZ] deficiency(RP1616),and the Control group(RP437).
 +
check the resistibility of RP1616 and RP437
 +
result:both of the two groups are of none resistibility.
 +
members:Siwei Luo, Fangzhou Zhao, Zhilin Chen...
 +
 +
2011.6.29
 +
----
 +
check the motility of RP1616 strain and RP437 strain
 +
cultivate Top10 strain
 +
result: the bacterial plaque of RP1616 is smaller than that of RP437, implying that RP1616 is of [http://partsregistry.org/Part:BBa_K557000 cheZ] deficiency, causing the decreased motility of the bacteria.
 +
 +
 +
6.30
 +
----
 +
prepare for the competent cell of Top10 strain cultivated on 6.29
 +
extract the genome of Top10 strain and use it as complates to run PCR of CheZ
 +
result: the concentration of PCR result is too low to continue the experiments.
 +
 +
7.1
 +
----
 +
conduct PCR of CheZ again
 +
ligate the CheZ DNA segment to plasmid PSb1C3, and transform it into Top10 competent cells
 +
result:as the picture shows.
 +
 +
7.2
 +
----
 +
pick up single colony from the Petri dish to reproduce, in which the competent cells are cultivated and use them colony PCR
 +
result:The bacteria grow up well on dishes with chloromycetin resistency，verifying that the transformation is successfull
 +
while the concentration of PCR product is pretty high,demonstrating that the plasmids indeed contains the sagment CheZ.
<html>
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                    <div class="title_icon"><img src="https://static.igem.org/mediawiki/2011/0/07/USTC-China_checked.gif" alt="" title="" /></div>
+
-
                        <h2> <span class="dark_blue">Modeling</span></h2>
+
-
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                        <div class="box_text">
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                      </html>[[File:A10 t1000.png|100px|center]] <html>Here we present modeling of our system in order to verify our design and help constructing new systems. Besides we have detailed analysis of riboswitches invovled in our system.You can also find it under Dry Lab                        </div>
+
-
                        <a href="https://2011.igem.org/Team:USTC-China/Drylab/modeling" class="details">+ details</a>
+
-
                    </div>
+
-
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                    <div class="title_icon"><img src="https://static.igem.org/mediawiki/2011/0/07/USTC-China_checked.gif" alt="" title="" /></div>
+
-
                        <h2> <span class="dark_blue">Team</span></h2>
+
-
                    </div>
+
-
                   
+
-
                    <div class="box_text_content">
+
-
                 
+
-
                        <div class="box_text">
+
-
                  We are USTC-IGEMers from variant departments and grades and build our project together. We have a happy summer together and would like to share it~                        </div>
+
-
                        <a href="https://2011.igem.org/Team:USTC-China/Team" class="details">+ details</a>
+
-
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+
-
  </div>
+
-
</td>
+
-
<td>
+
-
<div class="box_content">
+
-
<div class="box_title">
+
-
                    <div class="title_icon"><img src="https://static.igem.org/mediawiki/2011/0/07/USTC-China_checked.gif" alt="" title="" /></div>
+
-
                        <h2> <span class="dark_blue">Human Practice</span></h2>
+
-
                    </div>
+
-
                   
+
-
                    <div class="box_text_content">
+
-
                   
+
-
                        <div class="box_text">
+
-
                        In our project, we have always been focusing biosafety and issued our own Biosafety Three Laws. Furthermor, we found speacail groups and conduct surveys and open days to get people informed of synthetic biology.                          </div>
+
-
                        <a href="https://2011.igem.org/Team:USTC-China/Human_practice" class="details">+ details</a>
+
-
                    </div>
+
-
  </div>
+
 +
<div class="bio" id="swlbio">
 +
<h1>July 3-July 9</h1>
</html>
</html>
 +
7.3
 +
----
 +
extract the plasmids from the reproduced bacteria and send it to check the base sequence
 +
result: the concentration of the plasmids extracted is about 500ng/uL
 +
 +
7.4
 +
----
 +
extract the standard parts LuxPR and Terminator from plate sent by iGEM registry 2010 and put them into Top10 strain by transformation
 +
(LuxPr: Plate 2,24C  Terminator : Plate 1,6O)
 +
result: the bacteria on the plate with Amp resistency grows well
 +
7.5
 +
----
 +
pick up single colony from the Petri dish to reproduce in the liquid culture medium
 +
 +
 +
7.6
 +
----
 +
extract plasmids containing LuxPR and Terminator and make enzyme digestion with EcoR1 and Spe1 in order to validate the existence of the two parts
 +
recieve part Toggle Switch and rbs-Ci-ter from PKU and conduct transformation
 +
 +
result: Due to the short length of LuxPR and Terminator, the result of the enzyme digestion is hard to examine by electrophoresis.
 +
 +
7.7
 +
----
 +
result: there are a few colonies with part Toggle switch grown while no colony of rbs-Ci-ter appeared.
 +
 +
7.8
 +
----
 +
pick up single colony with part Toggle switch .
 +
extract the standard parts LuxPR and Terminator from plate sent by iGEM registry 2011 and put them into Top10 strain by transformation
 +
 +
7.9
 +
----
 +
pick up single colony with LuxPR & terminator.
 +
extract plasmids containing toggle switch to conduct PCR.
 +
 +
process PCR with plasmids aptamer-CheZ
 +
result:as the picture shows.
 +
the base sequence of CheZ extracted before is proved right.
 +
 +
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+
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-
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+
<h1>July 10-July 16</h1>
-
 
+
</html>
-
<td>
+
7.10
 +
----
 +
ligate aptamer-CheZ with PSB1C3 plasmids
 +
 
 +
extract plasmids containing LuxPR and terminator
 +
result:the concentractions of plasmids LuxPR and terminator are both 600ng/ul
 +
 
 +
7.11
 +
----
 +
transform PSB1C3 containing aptamer-CheZ into bacteria and cultivate.
 +
7.12
 +
----
 +
carry out Double digestion of toggle switch and pSB1c3 and ligate them together.
 +
 
 +
pick up single colony from bacteria cultivated yesterday.
 +
 
 +
result: Both the location and the brightness of the electrophoretic bands correspond with our expectation.
 +
 
 +
7.14
 +
----
 +
transform the part rbs-ci-ter sent from PKU again into bacteria.
 +
check the colors of the colonies containing toggle switch.
 +
result: the ratio of red vs green is 8:25, verifying the function of toggle switch.
 +
 
 +
7.15
 +
----
 +
pick up the single colony from the plate yesterday and ten hours later extract the plasmids containing Ci-terminator.
 +
 
 +
 
 +
7.16
 +
----
 +
conduct PCR with the plasmids yesterday to reproduce rbs-ci-Term.
 +
result:failure
 +
 
 +
 
<html>
<html>
-
<form  id="loginform" action="user_add.php" method="post" name="form1">
+
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<div name="login">
+
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<p>123445          </p>
+
<div class="bio" id="fzzbio">
-
      <input type="radio" name="ch" value="1" checked="checked"/>1
+
<h1>July 17-July 23</h1>
-
      <input type="radio" name="ch" value="2" />2
+
7.18-7.21
-
      <input type="radio" name="ch" value="3" />3
+
----
-
      <input type="radio" name="ch" value="4" />4
+
ligate the standard part Terminator to the end of toggle switch
-
  <input type="radio" name="ch" value="5" />5</p>
+
result: we conduct a enzyme digestion with EcoR1 and Pst1 to the final ligation product and the electrophoresis result shows that the ligation is successful.
 +
<div class="clear"></div>
 +
</div>
-
<div>                      
+
<div class="bio" id="shlbio">
 +
<h1>July 24-July 30</h1>
 +
7.22-7.29
 +
----
 +
perform the following experiments: PCR of rbs-ci-ter, enzyme digestion(E,S),ligation with LuxPR(standard part from IGEM registry digested by E,X),tranformation,plasmids extraction.
 +
result: the concentration of plasmids with ligation gene is as high as ...
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+
<div class="clear"></div>
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+
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var Obj = document.getElementsByName("ch"); 
+
 
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+
<div class="bio" id="mlbio">
-
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+
<h1>July 31-Aug 6</h1>
-
+
7.30-8.3
-
+
----
-
}
+
PCR of LuxPR-rbs-ci-ter, enzyme digestion(E,S),ligation with LuxPR(standard part from IGEM registry digested by E,X),tranformation,plasmids extraction.
-
</script>
+
</div>
-
</form>
+
 
 +
 
 +
<div class="bio" id="mpsbio">
 +
<h1>Aug 7-Aug 13</h1>
 +
 
 +
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 +
</div>
 +
 
 +
 
 +
<div class="bio" id="yflbio">
 +
<h1>Aug 14-Aug 20</h1>
 +
 
 +
</div>
 +
 
 +
 
 +
<div class="bio" id="nwbio">
 +
<h1></h1>
 +
 
 +
 
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 +
<h1></h1>
 +
 
 +
 
 +
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 +
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 +
 
 +
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 +
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 +
 
 +
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 +
 
 +
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 +
<h1></h1>
 +
 
 +
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 +
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 +
 
 +
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 +
<h1></h1>
 +
 
 +
 
 +
 
 +
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 +
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 +
 
 +
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 +
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 +
 
 +
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 +
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 +
 
 +
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 +
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 +
 
 +
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 +
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 +
 +
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 +
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 +
 
 +
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 +
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Revision as of 02:59, 29 October 2011


Weeks

  • Jun 28-July 2
  • July 3-July 9
  • July 10-July 16
  • July 17-July 23
  • July 24-July 30
  • July 31-Aug 6
  • Aug 7-Aug 13
  • Aug 14-Aug 20
  • Aug 21-Aug 27
  • Aug 28-Sep 3
  • Sep 4-Sep 10
  • Sep 11-Sep 17
  • Sep 18-Sep 24
  • Sep 25-Oct 1
  • Oct 2-Oct 8
  • Oct 9-Oct 15

Jun 28-July 2

2011.6.28

cultivate the bacteria of [http://partsregistry.org/Part:BBa_K557000 cheZ] deficiency(RP1616),and the Control group(RP437). check the resistibility of RP1616 and RP437 result:both of the two groups are of none resistibility. members:Siwei Luo, Fangzhou Zhao, Zhilin Chen...

2011.6.29

check the motility of RP1616 strain and RP437 strain cultivate Top10 strain result: the bacterial plaque of RP1616 is smaller than that of RP437, implying that RP1616 is of [http://partsregistry.org/Part:BBa_K557000 cheZ] deficiency, causing the decreased motility of the bacteria.


6.30

prepare for the competent cell of Top10 strain cultivated on 6.29 extract the genome of Top10 strain and use it as complates to run PCR of CheZ result: the concentration of PCR result is too low to continue the experiments.

7.1

conduct PCR of CheZ again ligate the CheZ DNA segment to plasmid PSb1C3, and transform it into Top10 competent cells result:as the picture shows.

7.2

pick up single colony from the Petri dish to reproduce, in which the competent cells are cultivated and use them colony PCR result:The bacteria grow up well on dishes with chloromycetin resistency，verifying that the transformation is successfull while the concentration of PCR product is pretty high,demonstrating that the plasmids indeed contains the sagment CheZ.


July 3-July 9

7.3

extract the plasmids from the reproduced bacteria and send it to check the base sequence result: the concentration of the plasmids extracted is about 500ng/uL

7.4

extract the standard parts LuxPR and Terminator from plate sent by iGEM registry 2010 and put them into Top10 strain by transformation (LuxPr: Plate 2,24C Terminator : Plate 1,6O) result: the bacteria on the plate with Amp resistency grows well 7.5

pick up single colony from the Petri dish to reproduce in the liquid culture medium


7.6

extract plasmids containing LuxPR and Terminator and make enzyme digestion with EcoR1 and Spe1 in order to validate the existence of the two parts recieve part Toggle Switch and rbs-Ci-ter from PKU and conduct transformation

result: Due to the short length of LuxPR and Terminator, the result of the enzyme digestion is hard to examine by electrophoresis.

7.7

result: there are a few colonies with part Toggle switch grown while no colony of rbs-Ci-ter appeared.

7.8

pick up single colony with part Toggle switch . extract the standard parts LuxPR and Terminator from plate sent by iGEM registry 2011 and put them into Top10 strain by transformation

7.9

pick up single colony with LuxPR & terminator. extract plasmids containing toggle switch to conduct PCR.

process PCR with plasmids aptamer-CheZ result:as the picture shows. the base sequence of CheZ extracted before is proved right.


July 10-July 16

7.10

ligate aptamer-CheZ with PSB1C3 plasmids

extract plasmids containing LuxPR and terminator result:the concentractions of plasmids LuxPR and terminator are both 600ng/ul

7.11

transform PSB1C3 containing aptamer-CheZ into bacteria and cultivate. 7.12

carry out Double digestion of toggle switch and pSB1c3 and ligate them together.

pick up single colony from bacteria cultivated yesterday.

result: Both the location and the brightness of the electrophoretic bands correspond with our expectation.

7.14

transform the part rbs-ci-ter sent from PKU again into bacteria. check the colors of the colonies containing toggle switch. result: the ratio of red vs green is 8:25, verifying the function of toggle switch.

7.15

pick up the single colony from the plate yesterday and ten hours later extract the plasmids containing Ci-terminator.


7.16

conduct PCR with the plasmids yesterday to reproduce rbs-ci-Term. result:failure


July 17-July 23

7.18-7.21 ---- ligate the standard part Terminator to the end of toggle switch result: we conduct a enzyme digestion with EcoR1 and Pst1 to the final ligation product and the electrophoresis result shows that the ligation is successful.

July 24-July 30

7.22-7.29 ---- perform the following experiments: PCR of rbs-ci-ter, enzyme digestion(E,S),ligation with LuxPR(standard part from IGEM registry digested by E,X),tranformation,plasmids extraction. result: the concentration of plasmids with ligation gene is as high as ...

July 31-Aug 6

7.30-8.3 ---- PCR of LuxPR-rbs-ci-ter, enzyme digestion(E,S),ligation with LuxPR(standard part from IGEM registry digested by E,X),tranformation,plasmids extraction.

Aug 7-Aug 13

Aug 14-Aug 20

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