Team:HokkaidoU Japan/Protocols/Primers

From 2011.igem.org

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=Primers=
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==Note==
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|style="width:61px;"|Forward||style="width:306px; text-align:left;"|SlrP coding sequence, from 373bp to 394 bp||style="width:51px;"|63.4 C||style="width:306px; text-align:left;"|None
|style="width:61px;"|Forward||style="width:306px; text-align:left;"|SlrP coding sequence, from 373bp to 394 bp||style="width:51px;"|63.4 C||style="width:306px; text-align:left;"|None
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Latest revision as of 14:13, 23 October 2011

Contents

Primers

Note

Primer Name Whole Sequence
F/RAnnealing SequenceTmAdding Sequence


General Primers

EX_F gcagaattcgcggccgcttctagag
ForwardBiobrick Prefix74.5 CNone
PS_R agcctgcagcggccgctactagta
ReverseBiobrick Suffix74.6 CNone
suffix_F tactagtagcggccgctgcaggct
ForwardBiobrick Suffix74.6 CNone
prefix_R ctctagaagcggccgcgaattctgc
ReverseBiobrick Prefix74.5 CNone
100up_EX_F aacctataaaaataccgcatacac
Forward100bp upstream from Biobrick prefix62.7 CNone
200dn_PS_R tcccctgattctgtggataaccgt
Reverse200bp downstream from Biobrick suffix66.6 CNone
  • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K496000 See details of 100up_EX_F/200dn_PS_R] (partsregistry)


Primers Used for Backbone Construction

Xba_byebye_prefix_F gcagaattcgcggccgcttcGagatg
ForwardBiobrick Prefix sequence72.8 CSingle mutation T -> G in XbaI site.
EX_RBS_SlrP_F GCAGAATTCGCGGCCGCTTCTAGAaaagaggagaaaatatgtttaatattactaatatacaatctacggc
ForwardRBS sequence, 5' terminal of SlrP coding sequence69.7 CBiobrick Prefix
PS_SlrP_R AGCCTGCAGCGGCCGCTACTAGTggtaagtcctaatattttcagacgaag
Reverse3'terminal of SlrP coding sequence64.5 CBiofusion Suffix
Bsa1_dt_F GGCGACTAGAGAGACCccaggcatcaaataaaacgaaag
Forward5'terminal of double terminator sequence63.6 CBsaI recognition site and its cleavage site
Bsa1_SlrP_R GGCCTGGCCTGAGACCCCggtaagtcctaatattttcagacga
Reverse3'terminal of SlrP coding Sequence63.0 CBsaI recognition site and its cleavage site
Bsa1_GSK_SlrP_R GGCCTGGCCTGAGACCCCACTTTCAGCGAAACTTGTAGTGCGAGGGCGACCACTCATggtaagtcctaatattttcagacga
Reverse3'terminal of SlrP coding Sequence63.6 CGSK Tag, BsaI recognition site and its cleavage site

Sequencing Primer

SlrP_373_to_394_F gaaagtcagtcacctatacccg
ForwardSlrP coding sequence, from 373bp to 394 bp63.4 CNone
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