Team:Freiburg/Description

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(Precipitator)
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==<span style="color:grey;">Precipitator</span>==
==<span style="color:grey;">Precipitator</span>==
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[[File:Freiburg11_Precipitator_scheme.png|right|caption|200px|Precipitator binding polystyrene surface with the plastic binding domain and a His-tagged Protein via Nickel]]
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[[File:Freiburg11_Precipitator_scheme.png|right|thumb|caption|200px|Precipitator binding polystyrene surface with the plastic binding domain and a His-tagged Protein via Nickel]]
We created a cellular, self-replicating purification device for His-tagged proteins. It is a completely artificial fusion protein, which consists of a repeating LRRNT motif domain, coordinating Ni2+ Ions on its surface capped on N and C terminal ends by a hagfish sequence of a similar LRRNT motif. A second domain, a short hydrophobic peptide stretch, binds a polystyrene surface. We named this construct “THE PRECIPITATOR”.
We created a cellular, self-replicating purification device for His-tagged proteins. It is a completely artificial fusion protein, which consists of a repeating LRRNT motif domain, coordinating Ni2+ Ions on its surface capped on N and C terminal ends by a hagfish sequence of a similar LRRNT motif. A second domain, a short hydrophobic peptide stretch, binds a polystyrene surface. We named this construct “THE PRECIPITATOR”.
After expression of the Precipitator in a light inducible E. coli strain, the cells are lysed and the lysate is taken up by a serological pipette, in preparation of the actual protein purification steps.
After expression of the Precipitator in a light inducible E. coli strain, the cells are lysed and the lysate is taken up by a serological pipette, in preparation of the actual protein purification steps.

Revision as of 15:29, 16 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!