Team:Wageningen UR/Project/Devices

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The micro-dish contains porous wells, in which the cells can be physically constrained. It is placed in the socket of the dish, which allows a fluid to be flown over the wells. This fluid can be used to supply the cells with nutrients and wash away excess AHL and cells if necessary. Figure 2 shows a schematic of the device. The inflow and outflow1 can be used to flush the device with fresh medium. The use of outflow2 depends on the bacteria growing platform. In the case of the micro-sieve it can be used to create an under pressure, e.g. with a syringe, to trap the bacteria on the sieve. T
The micro-dish contains porous wells, in which the cells can be physically constrained. It is placed in the socket of the dish, which allows a fluid to be flown over the wells. This fluid can be used to supply the cells with nutrients and wash away excess AHL and cells if necessary. Figure 2 shows a schematic of the device. The inflow and outflow1 can be used to flush the device with fresh medium. The use of outflow2 depends on the bacteria growing platform. In the case of the micro-sieve it can be used to create an under pressure, e.g. with a syringe, to trap the bacteria on the sieve. T
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[[File:Scheme_microsieve_WUR.png]]
[[File:Production_device_WUR.jpg|200px]]  
[[File:Production_device_WUR.jpg|200px]]  
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''' Fig.2:''' ''Production of the device in the Wageningen University workshop''
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''' Fig.3:''' ''Production of the device in the Wageningen University workshop''
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Revision as of 22:10, 16 September 2011

Building a Synchronized Oscillatory System

Fluid device designed by Team Wageningen UR to measure oscillations

The [http://www.nature.com/nature/journal/v463/n7279/abs/nature08753.html paper] our system is based on used microfluidic devices to measure oscillations. Such microfluidic devices however are very expensive and our iGEM team budget was limited, so we decided to design a customary fluidic device, which was produced in the Wageningen University workshop. To keep as many options as possible open, the design implemented the idea that the device can potentially accommodate two bacteria growing platforms, a micro-sieve and a micro-dish. Gaining more in-depth understanding of our system, we decided to focus on the micro-dish as platform to test the oscillatory behaviour of our construct. Figure 1 and 2 below show the the flow chamber as original scetch designed by our team and the 3D rendered depiction of the final device.


Scetch device WUR.jpg Module-2 WUR.JPG

Fig.1: Micro-sieve design: original scetch and 3D render

The micro-dish contains porous wells, in which the cells can be physically constrained. It is placed in the socket of the dish, which allows a fluid to be flown over the wells. This fluid can be used to supply the cells with nutrients and wash away excess AHL and cells if necessary. Figure 2 shows a schematic of the device. The inflow and outflow1 can be used to flush the device with fresh medium. The use of outflow2 depends on the bacteria growing platform. In the case of the micro-sieve it can be used to create an under pressure, e.g. with a syringe, to trap the bacteria on the sieve. T

Scheme microsieve WUR.png

Production device WUR.jpg Fig.3: Production of the device in the Wageningen University workshop






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