Team:Washington/Protocols
From 2011.igem.org
(Difference between revisions)
(7 intermediate revisions not shown) | |||
Line 3: | Line 3: | ||
<center><big><big><big><big>'''Protocols'''</big></big></big></big></center><br><br> | <center><big><big><big><big>'''Protocols'''</big></big></big></big></center><br><br> | ||
+ | |||
+ | =General Protocols= | ||
[https://2011.igem.org/Team:Washington/Protocols/gel_electrophoresis General Agarose Gel Electrophoresis] | [https://2011.igem.org/Team:Washington/Protocols/gel_electrophoresis General Agarose Gel Electrophoresis] | ||
Line 18: | Line 20: | ||
[https://2011.igem.org/Team:Washington/Protocols/Competent Competent Cell Prep Protocol] | [https://2011.igem.org/Team:Washington/Protocols/Competent Competent Cell Prep Protocol] | ||
- | [https://2011.igem.org/Team:Washington/Protocols/Kunkel Kunkel | + | [https://2011.igem.org/Team:Washington/Protocols/Kunkel Kunkel Mutagenesis] |
- | [http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of | + | [http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of the Kunkel Mutagenesis process] |
[https://2011.igem.org/Team:Washington/Protocols/expression_purification Standard 1L Expression Purification] | [https://2011.igem.org/Team:Washington/Protocols/expression_purification Standard 1L Expression Purification] | ||
Line 50: | Line 52: | ||
[https://2011.igem.org/Team:Washington/Protocols/Purified_Enzyme_Assay Enzyme Assay] | [https://2011.igem.org/Team:Washington/Protocols/Purified_Enzyme_Assay Enzyme Assay] | ||
- | =Make It: | + | |
+ | =Make It: iGEM Toolkits= | ||
[https://2011.igem.org/Team:Washington/Protocols/Cyto. Cytometry Protocol] | [https://2011.igem.org/Team:Washington/Protocols/Cyto. Cytometry Protocol] | ||
Line 65: | Line 68: | ||
[https://2011.igem.org/Team:Washington/Protocols/Induc_studies. Induction Studies of Proteins Fusions (mam-sfGFP)] | [https://2011.igem.org/Team:Washington/Protocols/Induc_studies. Induction Studies of Proteins Fusions (mam-sfGFP)] | ||
+ | |||
+ | [https://2011.igem.org/Team:Washington/Protocols/pGA. pGA Vector Assay] | ||
[https://2011.igem.org/Team:Washington/Protocols/PBS. PBS Stock Protocol] | [https://2011.igem.org/Team:Washington/Protocols/PBS. PBS Stock Protocol] | ||
[https://2011.igem.org/Team:Washington/Protocols/Overnights. Preparation of Overnight Cultures] | [https://2011.igem.org/Team:Washington/Protocols/Overnights. Preparation of Overnight Cultures] | ||
+ | |||
=Wiki Design= | =Wiki Design= | ||
[https://2011.igem.org/Team:Washington/Protocols/Wiki_Design Wiki Design Tools (Wiki Markup, WikiDust, etc.)] | [https://2011.igem.org/Team:Washington/Protocols/Wiki_Design Wiki Design Tools (Wiki Markup, WikiDust, etc.)] |
Latest revision as of 02:10, 29 September 2011
General Protocols
General Agarose Gel Electrophoresis
General Transformation Protocol
[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of the Kunkel Mutagenesis process]
Standard 1L Expression Purification
Make It: Diesel Production Protocols
Alkane Biosynthesis media and extraction
Cell Lysate Assay by Decarbonylase Redesign Team
Break It: Gluten Destruction Protocols
Small Scale (50mL) Protein Expression and Purification
Make It: iGEM Toolkits
Electroporation (Transformation)
High-Yield PCR (Full-Gene Assembly)
Isolation of Plasmid DNA (miniprep)
Induction Studies of Proteins Fusions (mam-sfGFP)
Preparation of Overnight Cultures
Wiki Design
Wiki Design Tools (Wiki Markup, WikiDust, etc.)