Team:Wageningen UR/Project/DevicesMeasuringOscillations
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(→Customary fluidic device designed by Team Wageningen UR to measure oscillations) |
(→Customary fluidic device designed by Team Wageningen UR to measure oscillations) |
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- | == | + | == Custom fluidic device designed by Team Wageningen UR to measure oscillations == |
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- | For the experiments, an overnight culture of the cells containing the relevant construct was spun down and resuspended in PBS. The resuspended culture was inoculated in the device and | + | For the experiments, an overnight culture of the cells containing the relevant construct was spun down and resuspended in PBS. The resuspended culture was inoculated in the device and left in the chamber to settle down for a while. Since the bacteria were bottom fed with LB as seen in the set up section in [[Team:Wageningen_UR/Project/DevicesSetup#Controlling_cell_growth| figure X+2]], only the bacteria which settled down in the wells survived, while the bacteria in PBS starved to death. This is shown in the short video below. The pictures were taken every ten minutes. |
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- | For the experiment seen above, the PBS was removed before all the cells died. | + | For the experiment seen above, the PBS was removed before all the cells died. The procedure varied depending on how well the bacteria grew in the wells. They were left to grow in the device for an additional night and the measurements were then taken in an 10 minute intervall during the next day. This was done for two reasons, for one the chamber had to be completely dried out before measurements could be taken, otherwise the remaining liquid would condense through the heat of the light and blurr the pictures. The second reason was that, as mentioned before, [[Team:Wageningen_UR/Project/ModelingProj1#Conclusions_for_our_system| modeling]] suggested that, when applying no flow, the oscillations would occur only when starting with a high cell density. |
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Latest revision as of 16:09, 19 September 2011