Team:Washington/Protocols
From 2011.igem.org
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{{Template:Team:Washington/Templates/Top}} | {{Template:Team:Washington/Templates/Top}} | ||
__NOTOC__ | __NOTOC__ | ||
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+ | <center><big><big><big><big>'''Protocols'''</big></big></big></big></center><br><br> | ||
=General Protocols= | =General Protocols= | ||
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[https://2011.igem.org/Team:Washington/Protocols/Competent Competent Cell Prep Protocol] | [https://2011.igem.org/Team:Washington/Protocols/Competent Competent Cell Prep Protocol] | ||
- | [https://2011.igem.org/Team:Washington/Protocols/Kunkel Kunkel | + | [https://2011.igem.org/Team:Washington/Protocols/Kunkel Kunkel Mutagenesis] |
- | [http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of | + | [http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of the Kunkel Mutagenesis process] |
[https://2011.igem.org/Team:Washington/Protocols/expression_purification Standard 1L Expression Purification] | [https://2011.igem.org/Team:Washington/Protocols/expression_purification Standard 1L Expression Purification] | ||
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[https://2011.igem.org/Team:Washington/alkanebiosynthesis Alkane Biosynthesis media and extraction] | [https://2011.igem.org/Team:Washington/alkanebiosynthesis Alkane Biosynthesis media and extraction] | ||
- | [https://2011.igem.org/Team:Washington/Protocols/redesign_cell_lysate_assay] | + | [https://2011.igem.org/Team:Washington/alkanebiosynthesis_cloning Alkane Biosynthesis cloning] |
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+ | [https://2011.igem.org/Team:Washington/Protocols/redesign_cell_lysate_assay Cell Lysate Assay by Decarbonylase Redesign Team] | ||
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=Break It: Gluten Destruction Protocols= | =Break It: Gluten Destruction Protocols= | ||
- | [https://2011.igem.org/Team:Washington/Protocols/Cell_Lysate_Assay Cell Lysate Assay] | + | [https://2011.igem.org/Team:Washington/Protocols/Cell_Lysate_Assay Whole Cell Lysate Assay] |
[https://2011.igem.org/Team:Washington/Protocols/50mL_Scale Small Scale (50mL) Protein Expression and Purification] | [https://2011.igem.org/Team:Washington/Protocols/50mL_Scale Small Scale (50mL) Protein Expression and Purification] | ||
+ | [https://2011.igem.org/Team:Washington/Protocols/Purified_Enzyme_Assay Enzyme Assay] | ||
- | =Make It: | + | |
+ | =Make It: iGEM Toolkits= | ||
[https://2011.igem.org/Team:Washington/Protocols/Cyto. Cytometry Protocol] | [https://2011.igem.org/Team:Washington/Protocols/Cyto. Cytometry Protocol] | ||
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[https://2011.igem.org/Team:Washington/Protocols/Plas_DNA. Isolation of Plasmid DNA (miniprep)] | [https://2011.igem.org/Team:Washington/Protocols/Plas_DNA. Isolation of Plasmid DNA (miniprep)] | ||
- | [https://2011.igem.org/Team:Washington/Protocols/ | + | [https://2011.igem.org/Team:Washington/Protocols/Induc_studies. Induction Studies of Proteins Fusions (mam-sfGFP)] |
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+ | [https://2011.igem.org/Team:Washington/Protocols/pGA. pGA Vector Assay] | ||
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+ | [https://2011.igem.org/Team:Washington/Protocols/PBS. PBS Stock Protocol] | ||
[https://2011.igem.org/Team:Washington/Protocols/Overnights. Preparation of Overnight Cultures] | [https://2011.igem.org/Team:Washington/Protocols/Overnights. Preparation of Overnight Cultures] | ||
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=Wiki Design= | =Wiki Design= | ||
[https://2011.igem.org/Team:Washington/Protocols/Wiki_Design Wiki Design Tools (Wiki Markup, WikiDust, etc.)] | [https://2011.igem.org/Team:Washington/Protocols/Wiki_Design Wiki Design Tools (Wiki Markup, WikiDust, etc.)] |
Latest revision as of 02:10, 29 September 2011
General Protocols
General Agarose Gel Electrophoresis
General Transformation Protocol
[http://www.bio.davidson.edu/Courses/molbio/kunkel/kunkel.html Overview of the Kunkel Mutagenesis process]
Standard 1L Expression Purification
Make It: Diesel Production Protocols
Alkane Biosynthesis media and extraction
Cell Lysate Assay by Decarbonylase Redesign Team
Break It: Gluten Destruction Protocols
Small Scale (50mL) Protein Expression and Purification
Make It: iGEM Toolkits
Electroporation (Transformation)
High-Yield PCR (Full-Gene Assembly)
Isolation of Plasmid DNA (miniprep)
Induction Studies of Proteins Fusions (mam-sfGFP)
Preparation of Overnight Cultures
Wiki Design
Wiki Design Tools (Wiki Markup, WikiDust, etc.)