Team:Lyon-INSA-ENS/Realisation/ExperimentalSchedule

From 2011.igem.org

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<li style="margin-left:10%"> The first one is the construction of a part containing the OmpR234 curli regulator under the control of the cobalt inducible rcnA gene promotor.</li>
<li style="margin-left:10%"> The first one is the construction of a part containing the OmpR234 curli regulator under the control of the cobalt inducible rcnA gene promotor.</li>
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<li style="margin-left:10%"> The second one is the construction of a part containing the entire curli operon (csgBAEFG genes)under the control of the cobalt inducible rcnA gene promotor. </li>
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<li style="margin-left:10%"> The second one is the construction of a part containing the entire curli operon (csgBAEFG genes) under the control of the cobalt inducible rcnA gene promotor. </li>
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Revision as of 19:58, 22 August 2011










Experimental Schedule



The goal of the "Cobalt buster" project is to construct a bacterial strain which will be able to produce adherence structures (curlis) when cobalt is encountered in the liquid medium.


To make it possible we chose two strategies :


  • The first one is the construction of a part containing the OmpR234 curli regulator under the control of the cobalt inducible rcnA gene promotor.

  • The second one is the construction of a part containing the entire curli operon (csgBAEFG genes) under the control of the cobalt inducible rcnA gene promotor.
















  • ENS assystem Biomérieux INSA INSA