Team:Wageningen UR/Project/CompleteProject1Description
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====Introduction==== | ====Introduction==== | ||
The aim of this project is to design and implement a system exhibiting sustained oscillatory protein expression which should be visible and synchronized on the scale of a physically constrained population of E. coli cells. The principles that govern this type of behaviour have been studied both in theory and in practice, and as such there exists a solid foundation to apply these ideas in the context of the iGEM competition. In essence, this project consists of constructing a plasmid containing genes, the products of which reciprocally affect each other’s expression in a reliable manner. Based on a previously established design (REF), we intend to take advantage of the great variety of standardized, interchangeable and freely available Bio–Bricks (genes and regulatory elements; REF iGEM website) to construct modified genetic circuits, aiming at an improved bacterial oscillator. Due to the difficulty in experimentally verifying the phenomena we wish to observe, special considerations regarding the experimental set-up will have to be made. | The aim of this project is to design and implement a system exhibiting sustained oscillatory protein expression which should be visible and synchronized on the scale of a physically constrained population of E. coli cells. The principles that govern this type of behaviour have been studied both in theory and in practice, and as such there exists a solid foundation to apply these ideas in the context of the iGEM competition. In essence, this project consists of constructing a plasmid containing genes, the products of which reciprocally affect each other’s expression in a reliable manner. Based on a previously established design (REF), we intend to take advantage of the great variety of standardized, interchangeable and freely available Bio–Bricks (genes and regulatory elements; REF iGEM website) to construct modified genetic circuits, aiming at an improved bacterial oscillator. Due to the difficulty in experimentally verifying the phenomena we wish to observe, special considerations regarding the experimental set-up will have to be made. |
Revision as of 22:15, 1 August 2011
Building a Synchronized Oscillatory System
Synchronized Oscillatory System: Project Description