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Team:Wageningen UR/Notebook/Proj1/July
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Yesterday's liquid cultures were miniprepped. | Yesterday's liquid cultures were miniprepped. | ||
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| + | '''July 30''' | ||
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| + | Inoculated 500 mL erlenmeyers with 100 ml LB + 100 ug/ul. ''E. coli'' was grown overnight on LB + amp plates and directly used as inoculum. All cultures were done in duplo. ''E. coli'' strains used were: | ||
| + | ptet-GFP | ||
| + | R0062-GFP | ||
| + | F2621-GFP | ||
| + | RBS-GFP | ||
| + | |||
| + | The OD600 was measured 2 hours after inoculation and subsequently each hour, by taking 1 mL samples from the culture. the OD was measured with | ||
| + | Fluorescence was measured in a 96-wells plate on a fluorescent plate reader (Molecular Devices, M2 (toxicology). First blank measurements were done with 96 wells plates containing 50 ul LB+amp. The output corresponding with the specific wells was saved and used for further calculations. | ||
Revision as of 10:03, 30 July 2011
July - Synchronized Oscillatory System
- Home
- Team
- Project
- Safety
- Acknowledgements
- Media
- Outreach
July 13
BioBrick parts were digested using different combinations of restriction enzymes, ligated and run on a gel.
July 18
Gel extracion of the gel run on July 13th was performed. Liquid cultures were made.
July 19
Yesterday's liquid cultures were miniprepped.
July 30
Inoculated 500 mL erlenmeyers with 100 ml LB + 100 ug/ul. E. coli was grown overnight on LB + amp plates and directly used as inoculum. All cultures were done in duplo. E. coli strains used were: ptet-GFP R0062-GFP F2621-GFP RBS-GFP
The OD600 was measured 2 hours after inoculation and subsequently each hour, by taking 1 mL samples from the culture. the OD was measured with Fluorescence was measured in a 96-wells plate on a fluorescent plate reader (Molecular Devices, M2 (toxicology). First blank measurements were done with 96 wells plates containing 50 ul LB+amp. The output corresponding with the specific wells was saved and used for further calculations.
