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Team:Nevada/Notebook
From 2011.igem.org
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E. Coli | E. Coli | ||
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Results: Final NADH concentrations were determined using the molar extinction coefficient and were proportionate to initial glucose concentrations. | Results: Final NADH concentrations were determined using the molar extinction coefficient and were proportionate to initial glucose concentrations. | ||
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Discussion: Focus is now to measure not only glucose, but fructose concentrations as well as both with be secreted in the form of sucrose using a D-glucose/D-fructose assay (Megaenzyme. | Discussion: Focus is now to measure not only glucose, but fructose concentrations as well as both with be secreted in the form of sucrose using a D-glucose/D-fructose assay (Megaenzyme. | ||
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<a href="https://2011.igem.org/Team:Nevada/Notebook">Week 1</a> | <a href="https://2011.igem.org/Team:Nevada/Notebook">Week 1</a> | ||
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<a href="https://2011.igem.org/Team:Nevada/Notebook/June/Week2">Week 2</a> | <a href="https://2011.igem.org/Team:Nevada/Notebook/June/Week2">Week 2</a> | ||
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Latest revision as of 20:32, 30 June 2011
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E. Coli Cyano Enzymology Media
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E. Coli
Comment Here
Cyano
Comment Here
Hexokinase coupled assay used to quantitate amounts of cyanobacteria glucose secretion.
- Rxn:
- 1. glucose + ATP --Hx-> G-6-P + ADP
- 2. G-6-P + NAD+ --G-6-PDeH-> G-6-P + NADH
Reaction used Glucose assay kit (Genzyme) containing 2000U/L Hexokinase reagent and 4000U/L G-6-P DeH and measured glucose concentrations ranging from 0.05mM-0.25mM and absorbances were measured at 340.0 nm.
Media
