Team:Nevada/Notebook

From 2011.igem.org

(Difference between revisions)
m
m
Line 90: Line 90:
</div>
</div>
</div>
</div>
-
<!--
 
-
<font size="5">Week 2 - June 5-11
 
-
<br>
 
-
-----------------------------
 
-
</font>
 
-
<p><font color="red">E. Coli
 
-
<br>
 
-
<img src="https://static.igem.org/mediawiki/2011/a/a2/Growth_Curve_6_10_11.png" class="shadow" style="float:center"
 
-
height=400px width=400px>
 
-
<br>
 
-
<br>
 
-
<img src="https://static.igem.org/mediawiki/2011/b/bc/Log_Growth_Curve_6_10_11.png" class="shadow" style="float:center"
 
-
height=400px width=400px>
 
-
</font>
 
-
<p><font color="blue">Cyano</font>
 
-
<p><font color="green">
 
-
 
-
Assay for ethanol detection.  Simple one-step
 
-
assay takes ethanol and NAD+ into
 
-
acetylaldahyde and NADH with use of alcohol
 
-
Deh (ADH) enzyme.  Final NADH concentration
 
-
can be determined using its molar extinction
 
-
coefficient and absorbance at 340.0 nm, and
 
-
is proportionate to initial ethanol
 
-
concetration.
 
-
 
-
Ethanol concentrations ranging from 0.05mM-
 
-
0.25mM were assayed with 173U/mL ADH.
 
-
 
-
Results: Final NADH concentrations were
 
-
calculated to be off by a factor of ten due
 
-
to unexpectedly low absorbancies.  Assay was
 
-
repeated with increased ethanol concentrations
 
-
(0.5-10mM), but absorbancies remained lower
 
-
than expected.
 
-
 
-
Disscussion: It was determined that this assay
 
-
was now sensitive enough for the range of
 
-
ethanol concentrations that will need to be
 
-
detected in our project, therefore we will use
 
-
other means of ethanol detection using simple
 
-
primary alcohol detection methods using oxidizing reagents.</font>
 
-
 
-
<p><font color="black">Media</font>
 
-
<br>
 
-
 
-
<font size="5">Week 3- June 12-18
 
-
<br>
 
-
----------------------------------------------
 
-
</font>
 
-
<p><font color="red">E. Coli</font>
 
-
<p><font color="blue">Cyano</font>
 
-
<p><font color="green">Enzymology</font>
 
-
-->
 
</div>
</div>
</body>
</body>
</html>
</html>

Revision as of 19:30, 30 June 2011


Week 1 - June 1-4
-----------------------
E. Coli
Blah Blah Blah
Cyano
Blah Blah Blah
Hexokinase coupled assay used to quantitate amounts of cyanobacteria glucose secretion.
    Rxn:
  • 1. glucose + ATP --Hx-> G-6-P + ADP
  • 2. G-6-P + NAD+ --G-6-PDeH-> G-6-P + NADH

Reaction used Glucose assay kit (Genzyme) containing 2000U/L Hexokinase reagent and 4000U/L G-6-P DeH and measured glucose concentrations ranging from 0.05mM-0.25mM and absorbances were measured at 340.0 nm.
Week 1                                                                                                                                                         Week 2

Retrieved from "http://2011.igem.org/Team:Nevada/Notebook"