Team:Nevada/Notebook

From 2011.igem.org

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Revision as of 19:13, 30 June 2011

Week 1 - June 1-4
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E. Coli
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Cyano
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                             Hexokinase coupled assay used to quantitate                     amounts of cyanobacteria glucose secretion.
                             Rxn: 1. glucose + ATP --Hx-> G-6-P + ADP                     2. G-6-P + NAD+ --G-6-PDeH-> G-6-P + NADH

                             Reaction used Glucose assay kit (Genzyme)                     containing 2000U/L Hexokinase reagent and
                             4000U/L G-6-P DeH and measured glucose                     concentrations ranging from 0.05mM-0.25mM
                             and absorbances were measured at 340.0 nm.                     Results: Final NADH concentrations were
                             determined using the molar extinction                                 coefficient and were proportionate to intial
                             glucose concentrations.

                             Discussion: Focus is now to measure not only                     glucose, but fructose concentrations as well as
                             both with be secreted in the form of sucrose                     using a D-glucose/D-fructose assay (Megaenzyme)

Media

Week 1 Week 2

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